Simultaneous Quantification of 17 Cannabinoids in Cannabis Inflorescence by Liquid Chromatography-Mass Spectrometry

Separations

Sarah Purdy¹  Amitha K. Hewavitharana²  Bronwyn J. Barkla²  Tobias Kretzschmar²  Matthew Nolan²  Francine Gloerfelt-Tarp² 

[1] New South Wales Department of Primary Industries, 105 Prince Street, Orange, NSW 2800, Australia;Southern Cross Plant Science, Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia;

关键词: cannabinoids;    phytocannabinoids;    LC-MS;    Cannabis sativa;    tetrahydrocannabinol (THC);    cannabidiol (CBD);   

DOI  :  10.3390/separations9040085

来源: DOAJ

【 摘 要 】

With an increasing appreciation for the unique pharmacological properties associated with distinct, individual cannabinoids of Cannabis sativa, there is demand for accurate and reliable quantification for a growing number of them. Although recent methods are based on highly selective chromatography-mass spectrometry technology, most are limited to a few cannabinoids, while relying on unnecessarily sophisticated and expensive ultra-high performance liquid chromatography and tandem mass spectrometry. Here we report an optimised, simple extraction method followed by a reliable and simple high performance liquid chromatography method for separation. The detection is performed using a time-of-flight mass spectrometer that is available in most natural products research laboratories. Due to the simplicity of instrumentation, and the robustness resulting from a high resolution in the chromatography of isobaric cannabinoids, the method is well suited for routine phytocannabinoid analysis for a range of applications. The method was validated in terms of detection and quantification limits, repeatability, and recoveries for a total of 17 cannabinoids: detection limits were in the range 11–520 pg when using a 1 µL sample injection volume, and the recovery percentages ranged from 85% to 108%. The validated method was subsequently applied to determine cannabinoid composition in the inflorescences of several medicinal Cannabis sativa varieties.

【 授权许可】

Unknown