Hmga1/Hmga2 double knock-out mice display a “superpygmy” phenotype

Biology Open

Antonio Barbieri¹  Claudio Arra¹  Antonella Federico²  Floriana Forzati²  Giovanna Maria Pierantoni²  Francesco Esposito²  Alfredo Fusco²  Monica Fedele²  Dario Palmieri²  Ivana De Martino²  Giuseppe Palma² 

[1] Istituto Nazionale dei Tumori, Fondazione Pascale, 80131 Naples, Italy;Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Facoltà di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli “Federico II”, via Pansini 5, 80131 Naples, Italy;

关键词: HMGA1;    HMGA2;    Pygmy;    E2F;   

DOI  :  10.1242/bio.20146759

来源: DOAJ

【 摘 要 】

The HMGA1 and HMGA2 genes code for proteins belonging to the High Mobility Group A family. Several genes are negatively or positively regulated by both these proteins, but a number of genes are specifically regulated by only one of them. Indeed, knock-out of the Hmga1 and Hmga2 genes leads to different phenotypes: cardiac hypertrophy and type 2 diabetes in the former case, and a large reduction in body size and amount of fat tissue in the latter case. Therefore, to better elucidate the functions of the Hmga genes, we crossed Hmga1-null mice with mice null for Hmga2. The Hmga1−/−/Hmga2−/− mice showed reduced vitality and a very small size (75% smaller than the wild-type mice); they were even smaller than pygmy Hmga2-null mice. The drastic reduction in E2F1 activity, and consequently in the expression of the E2F-dependent genes involved in cell cycle regulation, likely accounts for some phenotypic features of the Hmga1−/−/Hmga2−/− mice.

【 授权许可】

Unknown