Characterization of Cancer Stem Cells in Colon Adenocarcinoma Metastasis to the Liver

Frontiers in Surgery

Helen D. Brasch¹  Shreeja Mehrotra¹  Tinte Itinteang¹  Hugo N. Humphries¹  Reginald W. Marsh²  Susrutha K. Wickremesekera³  Swee T. Tan⁴ 

[1] Gillies McIndoe Research Institute, Wellington, New Zealand;University of Auckland, Auckland, New Zealand;Upper Gastrointestinal, Hepatobiliary and Pancreatic Section, Department of General Surgery, Wellington Regional Hospital, Wellington, New Zealand;Wellington Regional Plastic, Maxillofacial and Burns Unit, Hutt Hospital, Wellington, New Zealand;

关键词: cancer stem cells;    colorectal;    colon;    cancer;    adenocarcinoma;    liver;   

DOI  :  10.3389/fsurg.2017.00076

来源: DOAJ

【 摘 要 】

BackgroundFifty percent of colorectal cancer (CRC) patients develop liver metastasis. This study identified and characterized cancer stem cells (CSCs) within colon adenocarcinoma metastasis to the liver (CAML).Methods3,3-Diaminobenzidine immunohistochemical (IHC) staining was performed on nine CAML samples for embryonic stem cell (ESC) markers OCT4, SOX2, NANOG, c-Myc, and KLF4. Immunofluorescence (IF) IHC staining was performed to investigate coexpression of two markers. NanoString mRNA expression analysis and colorimetric in situ hybridization (CISH) were performed on four snap-frozen CAML tissue samples for transcript expression of these ESC markers. Cells stained positively and negatively for each marker by IHC and CISH staining were counted and analyzed.Results3,3-Diaminobenzidine IHC staining, and NanoString and CISH mRNA analyses demonstrated the expression of OCT4, SOX2, NANOG, c-Myc, and KLF4 within in all nine CAML samples, except for SOX2 which was below detectable levels on NanoString mRNA analysis. IF IHC staining showed the presence of a SOX2+/NANOG+/KLF4+/c-Myc+/OCT− CSC subpopulation within the tumor nests, and a SOX2+/NANOG+/KLF4+/c-Myc+/OCT4− CSC subpopulation and a SOX2+/NANOG+/KLF4+/c-Myc+/OCT4+ CSC subpopulation within the peritumoral stroma.ConclusionThe novel finding of three CSC subpopulations within CAML provides insights into the biology of CRC.

【 授权许可】

Unknown