Frontiers in Neurology | |
Multidimensional Approach Assessing the Role of Interleukin 1 Beta in Mesial Temporal Lobe Epilepsy | |
Renato O. Santos1 Mariana S. Silva-Alves1 Claudia V. Maurer-Morelli1 Rodrigo Secolin1 Patrícia G. Barbalho1 Iscia Lopes-Cendes1 Antonio L. Teixeira2 Fernando Cendes3 Clarissa L. Yasuda3 Marina K. M. Alvim3 Tonicarlo R. Velasco4 Americo C. Sakamoto4 Fábio Rogerio5 | |
[1] Brazilian Institute of Neuroscience and Neurotechnology, Campinas, Brazil;Department of Internal Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil;Department of Neurology, University of Campinas, Campinas, Brazil;Department of Neuroscience and Behavioral Science, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil;Department of Pathology, University of Campinas, Campinas, Brazil;Department of Translational Medicine, University of Campinas, Campinas, Brazil; | |
关键词: mesial temporal sclerosis; hippocampal atrophy; association study; gene expression; neuroinflammation; | |
DOI : 10.3389/fneur.2021.690847 | |
来源: DOAJ |
【 摘 要 】
We aimed to investigate the role of interleukin-1 beta (IL-1β) in the mechanisms underlying mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS). We assessed a cohort of 194 patients with MTLE+HS and 199 healthy controls. Patients were divided into those with positive and negative antecedent febrile seizures (FS). We used a multidimensional approach, including (i) genetic association with single nucleotide polymorphisms (SNPs) in the IL1B gene; (ii) quantification of the IL1B transcript in the hippocampal tissue of patients with refractory seizures; and (iii) quantification of the IL-1β protein in the plasma. We found a genetic association signal for two SNPs, rs2708928 and rs3730364*C in the IL1B gene, regardless of the presence of FS (adjusted p = 9.62e–11 and 5.14e–07, respectively). We found no difference between IL1B transcript levels when comparing sclerotic hippocampal tissue from patients with MTLE+HS, without FS, and hippocampi from autopsy controls (p > 0.05). Nevertheless, we found increased IL-1β in the plasma of patients with MTLE+HS with FS compared with controls (p = 0.0195). Our results support the hypothesis of a genetic association between MTLE+HS and the IL1B gene
【 授权许可】
Unknown