β2-Adrenergic Receptor-Mediated HIF-1α Upregulation Mediates Blood Brain Barrier Damage in Acute Cerebral Ischemia

Frontiers in Molecular Neuroscience

Wen-Cao Liu¹  Mengwei Wang²  Chun-Feng Liu²  Xianzhi Shen²  Xinyu Zhang²  Yanyun Sun³  Xiaona Wang³  Xinchun Jin³  Wenlan Liu⁴  Xi Chen⁵  Jie Liu⁶ 

[1] Department of Emergency, Shanxi Provincial People’s HospitalTaiyuan, China;Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and Institute of Neuroscience, Department of Neurology, The Second Affiliated Hospital of Soochow UniversitySuzhou, China;School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Yantai University, Ministry of EducationYantai, China;The Central Laboratory, Shenzhen Second People’s Hospital, Stem Cell Research Center, The First Affiliated Hospital of Shenzhen UniversityShenzhen, China;The People’s Hospital of Baoan ShenzhenShenzhen, China;Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of MedicineShanghai, China;

关键词: cerebral ischemia;    HIF-1α;    β2-AR;    blood brain barrier;    tight junction proteins;    matrix metalloproteinase;   

DOI  :  10.3389/fnmol.2017.00257

来源: DOAJ

【 摘 要 】

Disruption of the blood brain barrier (BBB) within the thrombolytic time window is an antecedent event to intracerebral hemorrhage in ischemic stroke. Our recent studies showed that 2-h cerebral ischemia induced BBB damage in non-infarcted area and secreted matrix metalloproteinase-2 (MMP-2) accounted for this disruption. However, the factors that affect MMP-2 secretion and regulate BBB damage remains unknown. Since hypoxia-inducible factor-1 alpha (HIF-1α) was discovered as a mater regulator in hypoxia, we sought to investigate the roles of HIF-1α in BBB damage as well as the factors regulating HIF-1α expression in the ischemic brain. in vivo rat middle cerebral artery occlusion (MCAO) and in vitro oxygen glucose deprivation (OGD) models were used to mimic ischemia. Pretreatment with HIF-1α inhibitor YC-1 significantly inhibited 2-h MCAO-induced BBB damage, which was accompanied by suppressed occludin degradation and vascular endothelial growth factor (VEGF) mRNA upregulation. Interestingly, β2-adrenergic receptor (β2-AR) antagonist ICI 118551 attenuated ischemia-induced BBB damage by regulating HIF-1α expression. Double immunostaining showed that HIF-1α was upregulated in ischemic neurons but not in astrocytes andendothelial cells. Of note, HIF-1α inhibition with inhibitor YC-1 or siRNA significantly prevented OGD-induced VEGF upregulation as well as the secretion of VEGF and MMP-2 in neurons. More importantly, blocking β2-AR with ICI 118551 suppressedHIF-1α upregulation in ischemic neurons and attenuated occludin degradation induced by the conditioned media of OGD-treatedneurons. Taken together, blockade of β2-AR-mediated HIF-1α upregulation mediates BBB damage during acute cerebral ischemia. These findings provide new mechanistic understanding of early BBB damage in ischemic stroke and may help reduce thrombolysis-related hemorrhagic complications.

【 授权许可】

Unknown