Clever Experimental Designs: Shortcuts for Better iPSC Differentiation

Cells

Ryota Yasui¹  Keisuke Sekine¹  Hideki Taniguchi¹ 

[1] Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan;

关键词: design of experiments (DOE);    induced pluripotent stem cell (iPSC);    embryonic stem cell (ESC);    mesenchymal stem cell (MSC);    hematopoietic stem cell (HSC);    Chinese hamster ovary (CHO) cell;   

DOI  :  10.3390/cells10123540

来源: DOAJ

【 摘 要 】

For practical use of pluripotent stem cells (PSCs) for disease modelling, drug screening, and regenerative medicine, the cell differentiation process needs to be properly refined to generate end products with consistent and high quality. To construct and optimize a robust cell-induction process, a myriad of cell culture conditions should be considered. In contrast to inefficient brute-force screening, statistical design of experiments (DOE) approaches, such as factorial design, orthogonal array design, response surface methodology (RSM), definitive screening design (DSD), and mixture design, enable efficient and strategic screening of conditions in smaller experimental runs through multifactorial screening and/or quantitative modeling. Although DOE has become routinely utilized in the bioengineering and pharmaceutical fields, the imminent need of more detailed cell-lineage specification, complex organoid construction, and a stable supply of qualified cell-derived material requires expedition of DOE utilization in stem cell bioprocessing. This review summarizes DOE-based cell culture optimizations of PSCs, mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and Chinese hamster ovary (CHO) cells, which guide effective research and development of PSC-derived materials for academic and industrial applications.

【 授权许可】

Unknown