Effect of Paper-Bagging on Apple Skin Patterning Associated with MdMYB10 Promoter Methylation

International Journal of Molecular Sciences

Hye Jeong Cho¹  A Reum Han¹  Cheol Choi¹ 

[1] College of Agriculture and Life Sciences, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea;

关键词: anthocyanin;    apple;    bag treatment;    DNA methylation;    MYB transcription factor;    skin patterning;   

DOI  :  10.3390/ijms23063319

来源: DOAJ

【 摘 要 】

Paper-bagging is an efficient method to maximize apple skin color, but a relationship between this technique and fruit skin patterning has not been demonstrated. Here, the ‘Fuji’ fruit with red-striped skin changed to red-blushed skin under re-exposure to light after bag treatment. Higher expression of MdMYB10, a transcription factor that regulates anthocyanin biosynthesis in apples, correlated with increased anthocyanin concentration in bag removal fruit. At the mature stage, a comparison of methylation status in the MdMYB10 promoter revealed that the methylation level in the region from −2585 to −2117 bp was reduced in bag removal fruit, especially for CHG context. It can be regulated by the downregulated expression of DNA methyltransferases such as MdMET, MdCMT, and MdDRM. Our results suggest that the bag removal treatment in this cultivar causes a change in skin patterning from striped to blushed pigmentation by inducing DNA demethylation of MdMYB10.

【 授权许可】

Unknown