Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice

International Journal of Molecular Sciences

Wenqin Song¹  Bo Wang¹  Xia Wang¹  Deshui Yu¹  Chunguo Wang¹  Xiangyan Han¹  Chengbin Chen¹  Xuelian Zheng²  Zhaohui Zhong²  Yong Zhang² 

[1] College of Life Sciences, Nankai University, Tianjin 300071, China;Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China;

关键词: rice;    nac transcription factor;    abiotic stresses;    crispr-cas9;    transcriptome analysis;   

DOI  :  10.3390/ijms21072288

来源: DOAJ

【 摘 要 】

Rice (Oryza sativa) responds to various abiotic stresses during growth. Plant-specific NAM, ATAF1/2, and CUC2 (NAC) transcription factors (TFs) play an important role in controlling numerous vital growth and developmental processes. To date, 170 NAC TFs have been reported in rice, but their roles remain largely unknown. Herein, we discovered that the TF OsNAC006 is constitutively expressed in rice, and regulated by H₂O₂, cold, heat, abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin (GA), NaCl, and polyethylene glycol (PEG) 6000 treatments. Furthermore, knockout of OsNAC006 using the CRISPR-Cas9 system resulted in drought and heat sensitivity. RNA sequencing (RNA-seq) transcriptome analysis revealed that OsNAC006 regulates the expression of genes mainly involved in response to stimuli, oxidoreductase activity, cofactor binding, and membrane-related pathways. Our findings elucidate the important role of OsNAC006 in drought responses, and provide valuable information for genetic manipulation to enhance stress tolerance in future plant breeding programs.

【 授权许可】

Unknown