HS-SPME Gas Chromatography Approach for Underivatized Acrylamide Determination in Biscuits

Foods

Cláudia P. Passos¹  Manuel A. Coimbra¹  Andreia C. M. Neto¹  Sílvia Petronilho¹  Dylan Torres¹  Sílvia M. Rocha¹  António F. Serôdio¹  Cláudia Nunes¹  Alisa Rudnitskaya²  Kristína Kukurová³  Zuzana Ciesarová³ 

[1] Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;Centre for Environmental and Marine Studies (CESAM), Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;National Agricultural and Food Center, Food Research Institute, 824 75 Bratislava, Slovakia;

关键词: acrylamide;    HS-SPME;    GC-MS;    ion extraction;    cookies;    mitigation;   

DOI  :  10.3390/foods10092183

来源: DOAJ

【 摘 要 】

Acrylamide (AA) is a food contaminant in thermally processed products that is object of tight control. A simple and easy-to-apply methodology for routine monitoring of AA levels in food products could allow producers to be players in the control of their own products. In this work, a simple methodology for AA quantification without derivatization was developed for biscuits, for which the benchmark levels recommended by EFSA are 350 µg/kg, and 150 µg/kg for biscuits for infants and young children. Headspace-solid phase microextraction (HS-SPME) was used in 120 mL screwed-cap vials with a carboxen/polydimetylsiloxane fiber, 4 g of biscuits, and 10 mL of water during 15 min at room temperature under stirring. The addition of 30 mL of propanol under stirring during 15 min at room temperature and 15 min at 60 °C was used to promote AA transfer to the headspace. The fiber exposure was 45 min. A gas chromatography-mass spectrometry analysis allowed to obtain an external calibration curve at m/z 71, with linearity R² > 0.99 and precision RSD < 9%. The detection and quantification limits were 27.4 µg/kg and 91.5 µg/kg, respectively. The methodology was successfully used in biscuits with lower AA amount, where mitigation strategies (asparaginase or pectate) were applied.

【 授权许可】

Unknown