Comprehensive Profiling of Microbiologically Induced CaCO3 Precipitation by Ureolytic Bacillus Isolates from Alkaline Soils

Microorganisms

Jovana Stanojev¹  Branimir Bajac¹  Sabina Kovač²  Elvira Tóth³  Siniša Markov⁴  Olja Šovljanski⁴  Ivan Ristić⁴  Dragoljub Cvetković⁴  Aleksandra Ranitović⁴  Ana Tomić⁴  Lato Pezo⁵ 

[1] BioSense Institute, University of Novi Sad, Dr Zorana Đinđića 1, 21000 Novi Sad, Serbia;Department of Crystallography and Mineralogy, Faculty of Mining and Geology, University of Belgrade, Đušina 7, 11000 Belgrade, Serbia;Department of Physics, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia;Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia;Institute of General and Physical Chemistry, Studenski Trg 12/V, 11000 Belgrade, Serbia;

关键词: MICP process;    kinetic study;    calcite;    vaterite;    MALDI-TOF identification;    Bacillus licheniformis;   

DOI  :  10.3390/microorganisms9081691

来源: DOAJ

【 摘 要 】

Microbiologically induced CaCO₃ precipitation (MICP) is a well-known bio-based solution with application in environmental, geotechnical, and civil engineering. The significance of the MICP has increased explorations of process efficiency and specificity via natural bacterial isolates. In this study, comprehensive profiling of five soil ureolytic Bacillus strains was performed through a newly formed procedure that involved six steps from selection and identification, through kinetic study, to the characterization of the obtained precipitates, for the first time. To shorten the whole selection procedure of 43 bioagents with the MICP potential, Standard Score Analysis was performed and five selected bacteria were identified as Bacillus muralis, B. lentus, B. simplex, B. firmus, and B. licheniformis by the MALDI-TOF mass spectrometry. Despite following the targeted activity, kinetic studies were included important aspects of ureolysis and the MICP such as cell concentration, pH profiling, and reduction in calcium ion concentration. At the final step, characterization of the obtained precipitates was performed using FTIR, XRD, Raman, DTA/TGA, and SEM analysis. Although all tested strains showed significant potential in terms of precipitation of calcite or calcite and vaterite phase, the main differences in the MICP behavior can be observed at the bacterial strain level. B. licheniformis showed favorable behavior compared to the reference Sporosarcina pasteurii DSM 33.

【 授权许可】

Unknown