Chemico-Pharmacological Screening of the Methanol Extract of Gynura nepalensis D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from in vitro, in vivo, and Computer-Aided Studies

Molecules

Mohammed Fazlul Kabir¹  Seong-Tshool Hong²  Md. Minarul Islam²  Rashedul Alam³  Nazim Uddin Emon⁴  Tapas Sarkar⁵  Mohammad Manjur Rahman⁵  Nishan Chakrabarty⁶  Md. Moklesur Rahman Sarker⁷  Hea-Jong Chung⁸  Safaet Alam⁹ 

[1] Department of Biology, Georgia State University, Atlanta, GA 30303, USA;Department of Biomedical Sciences, Institute for Medical Science, Jeonbuk National University Medical School, Jeonju 54907, Korea;Department of Pharmacology, Medical School, Jeonbuk National University, Jeonju 54896, Korea;Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh;Department of Pharmacy, Gono Bishwabidyalay, Dhaka 1344, Bangladesh;Department of Pharmacy, Jagannath University, Dhaka 1100, Bangladesh;Department of Pharmacy, State University of Bangladesh, Dhaka 1205, Bangladesh;Gwanju Center, Korea Basic Science Institute, Gwanju 61715, Korea;Pharmaceutical Sciences Research Division, Bangladesh Council of Scientific and Industrial Research Laboratories (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka 1205, Bangladesh;

关键词: Gynura nepalensis;    hepatoprotective;    antioxidant;    bioactive compound;    phytomedicine;    binding affinity;   

DOI  :  10.3390/molecules27113474

来源: DOAJ

【 摘 要 】

Gynura nepalensis D.C. (family: Asteraceae) has abundant uses in the alternative medicinal practice, and this species is commonly used in the treatment of diabetes, rheumatism, cuts or wounds, asthma, kidney stones, cough, urinary tract bleeding, gall bladder stones, hepatitis, diarrhea, hemorrhoids, constipation, vomiting, fertility problems, blood poisoning, septicemia, skin allergy, indigestion, high cholesterol levels, and so on. This study aims to investigate the hepatoprotective and antioxidant potential of the methanol extract of the Gynura nepalensis D.C. (GNME) along with chemical profiling with phytochemical screening. Moreover, prospective phytocompounds have been screened virtually to present the binding affinity of the bioactive components to the hepatic and oxidative receptors. In the hepatoprotective study, alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), and lipid peroxidation (LP) and total bilirubin (TB) have been assessed, and in the antioxidant study, the DPPH free radical scavenging, total antioxidant flavonoid, and phenolic contents were determined. Moreover, the molecular binding affinity of the bioactive component of the plant has been analyzed using PyRx AutoDock Vina, Chimera, and Discovery Studio software. The plant extract showed dose-dependent hepatoprotective potential (p < 0.05, 0.01, 0.001) as well as strong antioxidant properties. Moreover, hepatoprotective and antioxidant molecular docking studies revealed a result varying from −2.90 kcal/mol to −10.1 kcal/mol. 4,5-dicaffeoylquinic acid and chlorogenic acid revealed the highest binding affinity among the selected molecules. However, the plant showed portent antioxidant and hepatoprotective properties in the in vitro, in vivo, and in silico models, and it is presumed that the hepatoprotective properties of the plant extract have occurred due to the presence of the vast bioactive chemical compounds as well as their antioxidant properties. Therefore, advanced studies are recommended to elucidate the pharmacological properties of the plant extracts.

【 授权许可】

Unknown