In Vivo Healthy Benefits of Galacto-Oligosaccharides from Lupinus albus (LA-GOS) in Butyrate Production through Intestinal Microbiota

Biomolecules

Carmen M. Gurrola-Díaz¹  Pedro M. García-López²  Rocio Ivette Lopez-Roa³  Vidal Delgado-Rizo⁴  Lucila A. Godínez-Méndez⁴  Liliana Íñiguez-Gutiérrez⁴  Mary Fafutis-Morris⁴  José Sergio Zepeda-Nuño⁵  Natali Vega-Magaña⁵ 

[1] Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico;Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biologíco y Agropecuarias, Universidad de Guadalajara, Guadalajara 45200, Jalisco, Mexico;Departamento de Farmacobiología, Centro Universitaro de Ciencias Exactas e Ingenierias, Universidad de Guadalajara, Guadalajara 44430, Jalisco, Mexico;Departamento de Fisiología, CIINDE, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico;Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico;

关键词: Lupinus albus;    short chain fatty acids;    ulcerative colitis;    intestinal microbiota;    dysbiosis;    goblet cells;   

DOI  :  10.3390/biom11111658

来源: DOAJ

【 摘 要 】

Animal digestive systems host microorganism ecosystems, including integrated bacteria, viruses, fungi, and others, that produce a variety of compounds from different substrates with healthy properties. Among these substrates, α-galacto-oligosaccharides (GOS) are considered prebiotics that promote the grow of gut microbiota with a metabolic output of Short Chain Fatty Acids (SCFAs). In this regard, we evaluated Lupinus albus GOS (LA-GOS) as a natural prebiotic using different animal models. Therefore, the aim of this work was to evaluate the effect of LA-GOS on the gut microbiota, SCFA production, and intestinal health in healthy and induced dysbiosis conditions (an ulcerative colitis (UC) model). Twenty C57BL/6 mice were randomly allocated in four groups (n = 5/group): untreated and treated non-induced animals, and two groups induced with 2% dextran sulfate sodium to UC with and without LA-GOS administration (2.5 g/kg bw). We found that the UC treated group showed a higher goblet cell number, lower disease activity index, and reduced histopathological damage in comparison to the UC untreated group. In addition, the abundance of positive bacteria to butyryl-CoA transferase in gut microbiota was significantly increased by LA-GOS treatment, in healthy conditions. We measured the SCFA production with significant differences in the butyrate concentration between treated and untreated healthy groups. Finally, the pH level in cecum feces was reduced after LA-GOS treatment. Overall, we point out the in vivo health benefits of LA-GOS administration on the preservation of the intestinal ecosystem and the promotion of SCFA production.

【 授权许可】

Unknown