Clinical Metagenomic Next-Generation Sequencing for Diagnosis of Secondary Glaucoma in Patients With Cytomegalovirus-Induced Corneal Endotheliitis

Frontiers in Microbiology

Yang Zhou¹  Lingwei Shen²  Hongwei Zhou²  Hua Jiang³  Xiangjun Chen⁵  Ying Zhang⁵  Guannan Bai⁶  Lidan Hu⁶  Wei Wu⁷ 

[1] BGI PathoGenesis Pharmaceutical Technology Co., Ltd., Hangzhou, China;Department of Clinical Laboratory, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China;Department of Otolaryngology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China;Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, China;The Children’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of Medicine, Hangzhou, China;Zhejiang Provincial Key Lab of Ophthalmology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China;

关键词: secondary glaucoma;    cytomegalovirus;    metagenomic next-generation sequencing;    herpes simplex virus;    varicella-zoster virus;   

DOI  :  10.3389/fmicb.2022.940818

来源: DOAJ

【 摘 要 】

Glaucoma is the second leading cause of blindness globally. Growing scientific evidence indicated that inflammation of the trabecular meshwork induced by corneal endotheliitis could lead to secondary glaucoma. Cytomegalovirus (CMV) has been identified as the most common herpes virus in corneal endotheliitis patients. Early detection is critical in preventing endothelial cell loss, and patient management should vary based on different pathological factors. However, routine culture and real-time polymerase chain reaction (qPCR) have difficult in distinguishing whether CMV, Varicella Zoster Virus (VZV) or Herpes Simplex Virus (HSV) causes endothiliitis. This may result in inappropriate treatment, which may prolong or aggravate the status of disease. We compared the sensitivity and specificity of qPCR and Metagenomic Next-Generation Sequencing (mNGS) in the aqueous humor of patients with suspected CMV endotheliitis in this study. Our results showed that four out of 11 (36.4%) of our patients were positive for CMV by qPCR, whereas mNGS had a 100% detection rate of CMV. Our findings implied that mNGS could be a useful diagnostic tool for CMV-induced endotheliitis.

【 授权许可】

Unknown