Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear

The Plant Pathology Journal

Duck Hwan Park¹  Chang-Sik Oh²  Jungkum Park²  Gyu Min Lee³  Donghyuk Kim⁴ 

[1] Applied Biology Program, Division of Bioresource Sciences, Kangwon National University, Chuncheon 24341, Korea;Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea;Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea;School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea;

关键词: Bacteriophage;    black shoot blight;    Erwinia amylovora;    Erwinia pyrifoliae;    fire blight;   

DOI  :  10.5423/PPJ.NT.06.2018.0100

来源: DOAJ

【 摘 要 】

Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. Erwinia amylovora and E. pyrifoliae cause fire blight and black shoot blight disease in apple and pear, respectively. In this study, the bacteriophage phiEaP-8 was isolated from apple orchard soil and could efficiently and specifically kill both E. amylovora and E. pyrifoliae. This bacteriophage belongs to the Podoviridae family. Whole genome analysis revealed that phiEaP-8 carries a 75,929 bp genomic DNA with 78 coding sequences and 5 tRNA genes. Genome comparison showed that phiEaP-8 has only 85% identity to known bacteriophages at the DNA level. PhiEaP-8 retained lytic activity up to 50°C, within a pH range from 5 to 10, and under 365 nm UV light. Based on these characteristics, the bacteriophage phiEaP-8 is novel and carries potential to control both E. amylovora and E. pyrifoliae in apple and pear.

【 授权许可】

Unknown