Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics

Frontiers in Genetics

Shao-Qing Wen¹  Yi Ding¹  Xiaoying Ren¹  Chang Sun²  Panxin Du²  Yichen Tao²  Yiran Xu²  Jianxue Xiong²  Hailiang Meng²  Kongyang Zhu³  Chuan-Chao Wang³ 

[1] Institute of Archaeological Science, Fudan University, Shanghai, China;MOE Key Laboratory of Contemporary Anthropology, Department of Anthropology and Human Genetics, School of Life Sciences, Fudan University, Shanghai, China;State Key Laboratory of Cellular Stress Biology, School of Life Sciences, State Key Laboratory of Marine Environmental Science, Department of Anthropology and Ethnology, Institute of Anthropology, School of Sociology and Anthropology, Xiamen University, Xiamen, China;

关键词: MGISEQ-2000;    BGI tech;    ancient DNA;    paleogenomics;    population genetics;   

DOI  :  10.3389/fgene.2021.745508

来源: DOAJ

【 摘 要 】

The MGISEQ-2000 sequencer is widely used in various omics studies, but the performance of this platform for paleogenomics has not been evaluated. We here compare the performance of MGISEQ-2000 with the Illumina X-Ten on ancient human DNA using four samples from 1750BCE to 60CE. We found there were only slight differences between the two platforms in most parameters (duplication rate, sequencing bias, θ, δS, and λ). MGISEQ-2000 performed well on endogenous rate and library complexity although X-Ten had a higher average base quality and lower error rate. Our results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEQ-2000 can be an alternative platform for paleogenomics sequencing.

【 授权许可】

Unknown