New geographic records for Echinococcus canadensis in coyotes and moose from Nova Scotia, Canada

International Journal for Parasitology: Parasites and Wildlife

Michael Boudreau¹  David L. McRuer²  Temitope U. Kolapo³  Donald T. Stewart³  Jenna Marie Priest⁴  Gary Conboy⁴  Emily J. Jenkins⁵  Dave Shutler⁵  Jason W.B. Power⁶ 

[1] Corresponding author. Department of Biology, Acadia University, Wolfville, Nova Scotia, B4P 2R6, Canada.;;Department of Lands &Forestry – Wildlife Division, Kentville, Nova Scotia, B4N 4E5, Canada;Department of Biology, Acadia University, Wolfville, Nova Scotia, B4P 2R6, Canada;;Department of Lands &Parks Canada, Department of Pathology and Microbiology, Atlantic Veterinary College, Charlottetown, Prince Edward Island, C1A 4P3, Canada;

关键词: Cyclophyllidean taeniid tapeworms;    Echinococcosis;    Metacestodes;    Unilocular cysts;   

DOI  :  

来源: DOAJ

【 摘 要 】

Echinococcus spp. tapeworms can cause serious diseases in mammals, including humans. Within the E. granulosus species complex, metacestodes produce unilocular cysts that are responsible for cystic echinococcosis in animal intermediate hosts. Canids are definitive hosts, harbouring adult cestodes in their intestines. Adult E. canadensis were recovered from the small intestine of 1 of 262 coyotes (Canis latrans) from Nova Scotia, Canada. Subsequently, we found unilocular cysts in lungs and livers of 4 of 8 sympatric moose (Alces alces) from Cape Breton Island. DNA was extracted from three cysts using the Qiagen DNeasy Blood and Tissue kit and assayed by polymerase chain reaction (PCR) with primers (cest4 and cest5) for a 117-bp region of the small subunit of ribosomal RNA of E. granulosus sensu lato, and further validated as E. canadensis G8 using primers targeting nicotinamide adenosine dinucleotide dehydrogenase subunit 1 (ND1) and cytochrome c oxidase subunit 1 (CO1) mitochondrial genes. These are the first records of E. canadensis in any of the three Maritime provinces, which include Nova Scotia, New Brunswick, and Prince Edward Island. The parasite was thought to be absent in this region due to extirpation of wolves (Canis spp.) in the 1800s. These findings suggest that further wildlife surveillance and risk assessment is warranted.

【 授权许可】

Unknown