Phospholipase Cβ3 Expressed in Mouse DRGs is Involved in Inflammatory and Postoperative Pain

Journal of Pain Research

关键词: phospholipase cβ3;    inflammatory pain;    postoperative pain;    thermal hyperalgesia;    mechanical hyperalgesia;   

DOI  :  

来源: DOAJ

【 摘 要 】

Susumu Ide,1 Tomoyuki Kawamata,1,2 Kumiko Ishida,1 Satoshi Fuseya,1 Takashi Ishida,1 Yuki Sugiyama,1 Mikito Kawamata,1 Satoshi Tanaka1 1Department of Anesthesiology and Resuscitology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan; 2Department of Anesthesiology, Wakayama Medical University, Wakayama, JapanCorrespondence: Susumu Ide; Satoshi TanakaDepartment of Anesthesiology and Resuscitology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, JapanTel [phone]Fax [phone]Email [e-mail]; [e-mail]: Previous studies suggested that phospholipase Cβ 3 (PLCβ 3), which is a common downstream component in the signaling cascade, plays an important role in peripheral mechanisms of perception including nociception. However, detailed profiles of PLCβ 3-expressing dorsal root ganglion (DRG) neurons and involvement of PLCβ 3 in inflammatory and postoperative pain have not been fully investigated.Purpose: We evaluated neurochemical char0acteristics of PLCβ 3-expressing DRG neurons in mice and then we examined the effects of selective knockdown of PLCβ 3 expression in DRGs on inflammatory and postoperative pain.Methods: Male C57BL/6-strain mice were used. For the inflammatory model, each mouse received subcutaneous injection of complete Freund’s adjuvant (CFA) in the left hindpaw. For the postoperative pain model, a plantar incision was made in the left hindpaw. PLCβ 3 antisense oligodeoxynucleotide or PLCβ 3 mismatch oligodeoxynucleotide was intrathecally administered once a day for three consecutive days in each model. The time courses of thermal hyperalgesia and mechanical hyperalgesia were investigated. Changes in PLCβ 3 protein levels in DRGs were evaluated by Western blotting.Results: Immunohistochemical analysis showed that high proportion of the PLCβ 3-positive profiles were biotinylated isolectin B4-positive or transient receptor potential vanilloid subfamily 1-positive. PLCβ 3 protein level in DRGs during CFA-induced inflammation was comparable to that at baseline. Intrathecal administration of PLCβ 3 antisense oligodeoxynucleotide, which significantly suppressed PLCβ 3 expression in DRGs, did not affect pain thresholds in normal conditions but inhibited CFA-induced thermal and mechanical hyperalgesia both at the early and late phases compared to that in mismatch oligodeoxynucleotide-treated mice. Intrathecal administration of PLCβ 3 antisense oligodeoxynucleotide also inhibited surgical incision-induced thermal and mechanical hyperalgesia.Conclusion: Our results uncover a unique role of PLCβ 3 in the development and maintenance of inflammatory pain induced by CFA application and in those of surgical incision-induced pain, although PLCβ 3 does not play a major role in thermal nociception or mechanical nociception in normal conditions.Keywords: phospholipase Cβ 3, inflammatory pain, postoperative pain, thermal hyperalgesia, mechanical hyperalgesia

【 授权许可】

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