Serologically-Based Evaluation of Cross-Protection Antibody Responses among Different A(H1N1) Influenza Strains

Vaccines

Emanuele Montomoli¹  Serena Marchi¹  Ilaria Manini¹  Giacomo Lazzeri¹  ClaudiaMaria Trombetta¹  EdmondJ. Remarque²  Pietro Piu³  Fabrizio Biuso³  Otfried Kistner³  Alessandro Manenti³  Tommaso Carli³ 

[1] Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy;Department of Virology, Biomedical Primate Research Centre, 2288 Rijswijk, The Netherlands;VisMederi srl, 53100 Siena, Italy;

关键词: influenza virus;    H1N1;    immunity;    antigenic drift;   

DOI  :  10.3390/vaccines8040656

来源: DOAJ

【 摘 要 】

After the influenza H1N1 pandemic of 2009, the seasonal A/Brisbane/59/2007 strain was replaced by the A/California/07/2009 strain for the influenza virus vaccine composition. After several seasons with no indications on the occurrence of antigenic drift, A/Michigan/45/2015 was chosen as the H1N1 vaccine strain for the 2017/2018 season. Since the immune response to influenza is shaped by the history of exposure to antigenically similar strains, the potential cross-protection between seasonal human influenza vaccine strains and the emerging pandemic strains was investigated. Human serum samples were tested by hemagglutination inhibition and single radial hemolysis assays against A/Brisbane/59/2007, A/California/07/2009, and A/Michigan/45/2015 strains. Strong cross-reactions between A/California/07/2009 and A/Michigan/45/2015 strains were observed in 2009/2010, most likely induced by the start of the 2009 pandemic, and the subsequent post-pandemic seasons from 2010/2011 onward when A/California/07/2009 became the predominant strain. In the 2014/2015 season, population immunity against A/California/07/2009 and A/Michigan/45/2015 strains increased again, associated with strong cross-reactions. Whereas hemagglutination inhibition assay has a higher sensitivity for detection of new seasonal drift, the single radial hemolysis assay is an excellent tool for determining the presence of pre-existing immunity, allowing a potential prediction on the booster potential of influenza vaccines against newly emerging drifted strains.

【 授权许可】

Unknown