Multilocus Sequence Typing Reveals Extensive Genetic Diversity of the Emerging Fungal Pathogen Scedosporium aurantiacum

Frontiers in Cellular and Infection Microbiology

Jean-Philippe Bouchara¹  Sharon C. A. Chen²  Wieland Meyer⁴  Ian Arthur⁵  Johannes Rainer⁶  Monica A. Slavin⁷  Christopher H. Heath⁸  Kathrin Tintelnot⁹  Heidemarie Losert⁹  Michaela Lackner^1,10  Josef Kaltseis^1,10  Walter Buzina^1,11  Alex Kan^1,12  Carolina Firacative^1,12  Katharina Schwabenbauer^1,12  Felix Gilgado^1,12  Azian Harun^1,13  Sarimah Abdullah^1,13  Christopher C. Blyth^1,14  Mark Fraser^1,15  Sandrine Giraud^1,16  Haybrig Perdomo^1,17  Josep Guarro Artigas^1,17  José F. Cano Lira^1,17 

[1] &;Department of Microbiology &Infectious Diseases Department, Fiona Stanley Hospital, Murdoch;Infectious Diseases, Royal Perth Hospital, Perth;0Mycology Laboratory, Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine Western Australia, Perth, WA, Australia;1Institute of Microbiology, Leopold Franzens University Innsbruck, Innsbruck, Austria;2Peter MacCallum Cancer Centre and Sir Peter MacCallum Department of Oncology, Melbourne, VIC, Australia;3Department of Microbiology, PathWest Laboratory Medicine, Fiona Stanley Hospital, Murdoch;FG 16 Mycology, Robert Koch Institute, Berlin, Germany;Institute of Hygiene and Microbiology, Medical University Innsbruck, Innsbruck, Austria;Institute of Hygiene, Microbiology and Environmental Medicine, Medical University, Graz, Austria;Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Faculty of Medicine and Health, Sydney Medical School, Westmead Clinical School, Sydney Institute for Infectious Diseases, Westmead Hospital-Research and Education Network, Westmead Institute for Medical Research, University of Sydney, Sydney, NSW, Australia;School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu, Malaysia;Telethon Kids Institute and Medical School, University of Western Australia, Perth, WA, Australia;UK National Mycology Reference Laboratory, National Infection Service, Public Health England South-West, Bristol, United Kingdom;UNIV Angers, Université de Bretagne Occidentale, Centre Hospitalier Universitaire (CHU) d’Angers, Groupe d’Etude des Interactions Hôte-Pathogène (GEIHP), EA3142, Structure Fédérative de Recherche “Interactions Cellulaires et Applications Thérapeutiques (SFR ICAT), Angers, France;Unitat de Microbiologia, Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, Reus, Spain;

关键词: Scedosporium aurantiacum;    MLST (multilocus sequence typing);    genotyping;    geographical origins;    ecological context;    clinical association;   

DOI  :  10.3389/fcimb.2021.761596

来源: DOAJ

【 摘 要 】

Scedosporium spp. are the second most prevalent filamentous fungi after Aspergillus spp. recovered from cystic fibrosis (CF) patients in various regions of the world. Although invasive infection is uncommon prior to lung transplantation, fungal colonization may be a risk factor for invasive disease with attendant high mortality post-transplantation. Abundant in the environment, Scedosporium aurantiacum has emerged as an important fungal pathogen in a range of clinical settings. To investigate the population genetic structure of S. aurantiacum, a MultiLocus Sequence Typing (MLST) scheme was developed, screening 24 genetic loci for polymorphisms on a tester strain set. The six most polymorphic loci were selected to form the S. aurantiacum MLST scheme: actin (ACT), calmodulin (CAL), elongation factor-1α (EF1α), RNA polymerase subunit II (RPB2), manganese superoxide dismutase (SOD2), and β-tubulin (TUB). Among 188 global clinical, veterinary, and environmental strains, 5 to 18 variable sites per locus were revealed, resulting in 8 to 23 alleles per locus. MLST analysis observed a markedly high genetic diversity, reflected by 159 unique sequence types. Network analysis revealed a separation between Australian and non-Australian strains. Phylogenetic analysis showed two major clusters, indicating correlation with geographic origin. Linkage disequilibrium analysis revealed evidence of recombination. There was no clustering according to the source of the strains: clinical, veterinary, or environmental. The high diversity, especially amongst the Australian strains, suggests that S. aurantiacum may have originated within the Australian continent and was subsequently dispersed to other regions, as shown by the close phylogenetic relationships between some of the Australian sequence types and those found in other parts of the world. The MLST data are accessible at http://mlst.mycologylab.org. This is a joined publication of the ISHAM/ECMM working groups on “Scedosporium/Pseudallescheria Infections” and “Fungal Respiratory Infections in Cystic Fibrosis”.

【 授权许可】

Unknown