Gllac7 Is Induced by Agricultural and Forestry Residues and Exhibits Allelic Expression Bias in Ganoderma lucidum

Frontiers in Microbiology

Qinghua Huang¹  Lining Wang¹  Biao Hu¹  Qingfu Wang¹  Lei Liang¹  Xiaoxia Ding² 

[1] Guangdong Engineering Laboratory of Biomass High-Value Utilization, Guangdong Plant Fiber Comprehensive Utilization Engineering Technology Research and Development Center, Guangzhou Key Laboratory of Biomass Comprehensive Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China;Key Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China;

关键词: G. lucidum;    laccase;    agricultural and forestry residues;    transcriptome profile;    allelic expression bias;    zinc finger;   

DOI  :  10.3389/fmicb.2022.890686

来源: DOAJ

【 摘 要 】

Ganoderma lucidum has a wide carbon spectrum, while the expression profile of key genes relevant to carbon metabolism on different carbon sources has been seldom studied. Here, the transcriptomes of G. lucidum mycelia cultured on each of 19 carbon sources were conducted. In comparison with glucose, 16 to 1,006 genes were upregulated and 7 to 1,865 genes were downregulated. Significant gene expression dynamics and induced activity were observed in laccase genes when using agricultural and forestry residues (AFRs) as solo carbon sources. Furthermore, study of laccase gene family in two haploids of G. lucidum GL0102 was conducted. Totally, 15 and 16 laccase genes were identified in GL0102_53 and GL0102_8, respectively, among which 15 pairs were allelic genes. Gene structures were conserved between allelic laccase genes, while sequence variations (most were SNPs) existed. Nine laccase genes rarely expressed on all the tested carbon sources, while the other seven genes showed high expression level on AFRs, especially Gllac2 and Gllac7, which showed 5- to 1,149-fold and 4- to 94-fold upregulation in mycelia cultured for 5 days, respectively. The expression of H53lac7 was consistently higher than that of H8lac7_1 on all the carbon sources except XM, exhibiting a case of allelic expression bias. A total of 47 SNPs and 3 insertions/deletions were observed between promoters of H53lac7 and H8lac7_1, which lead to differences in predicted binding sites of zinc fingers. These results provide scientific data for understanding the gene expression profile and regulatory role on different carbon sources and may support further functional research of laccase.

【 授权许可】

Unknown