Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

International Journal of Molecular Sciences

Olga Khaziakhmatova¹  Larisa Litvinova¹  Igor Khlusov¹  Valeria Shupletsova¹  Vladimir Malashchenko¹  Natalia Todosenko¹  Kristina Yurova¹  Elena Melashchenko¹  Egor Shunkin¹  Marina Khlusova²  Yurii Sharkeev³  Ekaterina Komarova³  Maria Sedelnikova³ 

[1] Center for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236000, Russia;Department of Pathophysiology, Siberian State Medical University, Tomsk 634050, Russia;Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, SB RAS, Tomsk 634055, Russia;

关键词: human adipose tissue;    stem cell culture;    cytokine/chemokine genes;    osteogenic genes;    microarc oxidation technique;    technological coating properties;   

DOI  :  10.3390/ijms21207682

来源: DOAJ

【 摘 要 】

The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (RUNX2, BMP2, BMP6, BGLAP, and ALPL) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.

【 授权许可】

Unknown