Effect of Long-Term 3D Spheroid Culture on WJ-MSC

Cells

Marta Kot¹  Agnieszka Kaminska²  Aleksandra Wedzinska²  Anna Sarnowska² 

[1] Mossakowski Medical Research Centre, Department of Stem Cell Bioengineering, Polish Academy of Sciences, 02-106 Warsaw, Poland;Mossakowski Medical Research Centre, Translational Platform for Regenerative Medicine, Polish Academy of Science, 02-106 Warsaw, Poland;

关键词: mesenchymal stem cells;    mesenchymal stromal cells;    3D culture;    neurospheres;    spheroids;    pluripotency;   

DOI  :  10.3390/cells10040719

来源: DOAJ

【 摘 要 】

The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time in 3D conditions. We examined the effects of prolonged 3D spheroid culture on MSC in hope to select cells with primitive features. Wharton Jelly derived MSC (WJ-MSC) were cultured in 3D neurosphere induction medium for about 20 days in vitro. Then, cells were transported to 2D conditions and confront to the initial population and population constantly cultured in 2D. 3D spheroids culture of WJ-MSC resulted in increased senescence, decreased stemness and proliferation. However long-termed 3D spheroid culture allowed for selection of cells exhibiting increased expression of early neural and SSEA4 markers what might indicate the survival of cell subpopulation with unique features.

【 授权许可】

Unknown