Effect of Dimer Structure and Inhomogeneous Broadening of Energy Levels on the Action of Flavomononucleotide in Rigid Polyvinyl Alcohol Films

International Journal of Molecular Sciences

Ignacy Gryczyński¹  Hanna Grajek²  Agnieszka I. Piotrowicz-Cieślak³  Grażyna Żurkowska⁴  Piotr Bojarski⁵  Jerzy Karolczak⁶  Jacek Kubicki⁶ 

[1] Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center at Ft. Worth, 3500 Camp Bowie Blvd, Ft. Worth, TX 76107, USA;Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland;Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland;Faculty of Applied Physics and Mathematics, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland;Faculty of Mathematics, Physics and Informatics, Institute of Experimental Physics, University of Gdańsk, Wita Stwosza 57, 80-308 Gdańsk, Poland;Faculty of Physics, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland;

关键词: flavin mononucleotide (FMN);    nonradiative energy transfer (NET);    inhomogeneous orientational broadening of energy levels (IOBEL);    time-resolved spectroscopy;    fluorescence decays from dimer states;    fluorescent centers in biological systems;   

DOI  :  10.3390/ijms22147759

来源: DOAJ

【 摘 要 】

The results of time-resolved fluorescence measurements of flavin mononucleotide (FMN) in rigid polyvinyl alcohol films (PVA) demonstrate that fluorescence intensity decays are strongly accelerated in the presence of fluorescent dimers and nonradiative energy transfer processes. The fluorescence decay originating both from H and J dimer states of FMN was experimentally observed for the first time. The mean fluorescence lifetimes for FMN dimers were obtained: ${⟨\mathsf{\tau }⟩}_{fl}$ = 2.66 ns (at λ_exc = 445 nm) and ${⟨\mathsf{\tau }⟩}_{fl}$ = 2.02 (at λ_exc = 487 nm) at λ_obs = 600 nm and T = 253 K from H and J state of dimers, respectively. We show that inhomogeneous orientational broadening of energy levels (IOBEL) affects the shape of the fluorescence decay and leads to the dependence of the average monomer fluorescence lifetime on excitation wavelength. IOBEL affected the nonradiative energy transfer and indicated that different flavin positioning in the protein pocket could (1) change the spectroscopic properties of flavins due to the existence of “blue” and “red” fluorescence centers, and (2) diminish the effectiveness of energy transfer between FMN molecules.

【 授权许可】

Unknown