Effect of Different Substrates on Soil Microbial Community Structure and the Mechanisms of Reductive Soil Disinfestation

Frontiers in Microbiology

Hongkai Liao¹  Huaiying Yao³  Liangzuo Shu⁴  Xingyan Tan⁴ 

[1] Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China;Ningbo Key Lab of Urban Environment Process and Pollution Control, Ningbo Urban Environment Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Ningbo, China;Research Center for Environmental Ecology and Engineering, Wuhan Institute of Technology, Wuhan, China;School of Life Sciences, Huaibei Normal University, Key Laboratory of Plant Resources and Biology of Anhui Province, Huaibei, China;

关键词: reductive soil disinfestation;    tomato;    organic matter;    C/N ratio;    soil microbial community;   

DOI  :  10.3389/fmicb.2019.02851

来源: DOAJ

【 摘 要 】

Reductive soil disinfestation (RSD) has recently attracted much attention owing to its effectiveness for controlling pathogens. In this study, we aimed to evaluate the effects of different C/N substrates on RSD and to explore the changes in microbial community structure during RSD treatment. The experimental set up included 10 groups, as follows: CK, without substrates; RSD treatments with alfalfa (Medicago sativa L.)[AL], maize (Zea mays Linn. Sp.) straw [MS], and rice (Oryza sativa L.) straw [RS], with three levels of addition (0.5% [L], 2% [M], and 5% [H]), yielding ALL, ALM, ALH, MSL, MSM, MSH, RSL, RSM, and RSH groups. Compared with CK, RSD treatments significantly increased the content of NH4+-N, and effectively eliminated the accumulated NO3--N in the soil. The relative abundances of organic acid producers, including Clostridium, Coprococcus, and Oxobacter, in all RSD groups were significantly higher than those in the CK by day 21. Moreover, on day 21, Aspergillus and Fusarium in all RSD groups were significantly lower than those in the CK. In summary, RSD treatments clearly increased the relative abundances of organic acid generators and effectively inhibited pathogens; however, when the C/N was too low and the amount of addition too high, ammonia poisoning and rapid growth of some microorganisms (e.g., Pseudallescheria and Arthrographis) may occur.

【 授权许可】

Unknown