Prelude to a panzootic: Gene flow and immunogenetic variation in northern little brown myotis vulnerable to bat white-nose syndrome

FACETS

Michael E. Donaldson¹  Cori L. Lausen²  Kyle Ritchie³  Karen J. Vanderwolf⁴  Donald F. McAlpine⁴  Thomas S. Jung⁵  Yessica Rico⁶  Douglas W. Burles⁷  Craig K.R. Willis⁷  Scott McBurney⁸  Christopher J. Kyle⁹  Christina M. Davy^1,10  Kathleen Dogantzis^1,10  Allysia Park^1,11 

[1] Department of Biology, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB R3B 2E9, Canada;Forensic Science Department, Trent University, 2140 East Bank Drive, Peterborough, ON K9J 7B8, Canada;;Canadian Wildlife Health Cooperative, Atlantic Region, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI C1A 4P3, Canada;Department of Biology, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB R3B 2E9, Canada;Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9J 7B8, Canada;Forensic Science Department, Trent University, 2140 East Bank Drive, Peterborough, ON K9J 7B8, Canada;Gwaii Haanas National Park Reserve/Haida Heritage Site, P.O. Box 37, Queen Charlotte City, BC V0T 1S0, Canada;New Brunswick Museum, 277 Douglas Avenue, Saint John, NB E2K 1E5, Canada;Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Peterborough, ON K9J 7B8, Canada;Yukon Department of Environment, P.O. Box 2703, Whitehorse, YT Y1A 2C6, Canada;

关键词: biological invasions;    emerging infectious diseases (EIDs);    gene flow;    immunogenetics;    local adaptation;    selective sweep;   

DOI  :  10.1139/facets-2017-0022

来源: DOAJ

【 摘 要 】

The fungus that causes bat white-nose syndrome (WNS) recently leaped from eastern North America to the Pacific Coast. The pathogen’s spread is associated with the genetic population structure of a host (Myotis lucifugus). To understand the fine-scale neutral and immunogenetic variation among northern populations of M. lucifugus, we sampled 1142 individuals across the species’ northern range. We used genotypes at 11 microsatellite loci to reveal the genetic structure of, and directional gene flow among, populations to predict the likely future spread of the pathogen in the northwest and to estimate effective population size (Ne). We also pyrosequenced the DRB1-like exon 2 of the class II major histocompatibility complex (MHC) in 160 individuals to explore immunogenetic selection by WNS. We identified three major neutral genetic clusters: Eastern, Montane Cordillera (and adjacent sampling areas), and Haida Gwaii, with admixture at intermediate areas and significant substructure west of the prairies. Estimates of Ne were unexpectedly low (289–16 000). Haida Gwaii may provide temporary refuge from WNS, but the western mountain ranges are not barriers to its dispersal in M. lucifugus and are unlikely to slow its spread. Our major histocompatibility complex (MHC) data suggest potential selection by WNS on the MHC, but gene duplication limited the immunogenetic analyses.

【 授权许可】

Unknown