Synchrotron-based FTIR microspectroscopy of chili resistance induced by Bacillus subtilis strain D604 against anthracnose disease

Journal of Plant Interactions

Supaporn Kaewnum¹  Piyaporn Phansak²  Weravart Namanusart³  Kunthika Vechklang³  Toan Le Thanh⁴  Mathukorn Sompong⁴  Natthiya Buensanteai⁴  Darawadee Wongchalee⁴  Kanjana Thumanu⁵ 

[1] Bioactive Agro-Industry Co., Ltd, Seang-Sang;Nakhon Phanom University;Rajamangala University of Technology;School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology;Synchrotron Light Research Institute (Public Organization);

关键词: SR-Fourier transform infrared;    chili anthracnose;    Bacillus subtilis;    biochemical composition;    hierarchical cluster analysis (HCA);    induced resistance;   

DOI  :  10.1080/17429145.2017.1325523

来源: DOAJ

【 摘 要 】

The aim of this study was to determine the resistance mechanisms of chili induced by the Bacillus subtilis strain D604 using synchrotron FTIR microspectroscopy (SR-FTIR). In this study, the strain D604 reduced anthracnose disease severity in chili plants by approximately 31.10%. The SR-FTIR spectral changes from the epidermis and mesophyll leaf tissue revealed higher integral areas for the C=O ester from lipids, lignin, or pectin (1770–1700 cm−1) as well as polysaccharides (1200–900 cm−1) in the treated samples of D606 and distilled water and then challenge inoculation with chili anthracnose pathogen, Colletotrichum acutatum. The secondary structure of the Amide I protein failed to convert from alpha helices (centered at 1650 cm−1) to beta sheets (centered at 1600 cm−1) in the mesophyll of samples not treated with D604. This study suggested that the strain D604 induced resistance against anthracnose pathogen in chili by inducing cellular changes related to defense compounds involved in plant defense mechanism.

【 授权许可】

Unknown