Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

International Journal of Molecular Sciences

Mary Jane Espina¹  Ryan Merry¹  Robert M. Stupar¹  Aaron J. Lorenz¹  Michael J. Morrisey²  Michelle A. Graham²  Jamie A. O’Rourke² 

[1] Department of Genetics and Agronomy, University of Minnesota, St. Paul, MN 55108, USA;USDA-ARS, CICGRU, Ames, IA 50011, USA;

关键词: soybean;    iron deficiency;    RNA-seq;    virus induced gene silencing (VIGS);   

DOI  :  10.3390/ijms222011032

来源: DOAJ

【 摘 要 】

The soybean (Glycine max L. merr) genotype Fiskeby III is highly resistant to a multitude of abiotic stresses, including iron deficiency, incurring only mild yield loss during stress conditions. Conversely, Mandarin (Ottawa) is highly susceptible to disease and suffers severe phenotypic damage and yield loss when exposed to abiotic stresses such as iron deficiency, a major challenge to soybean production in the northern Midwestern United States. Using RNA-seq, we characterize the transcriptional response to iron deficiency in both Fiskeby III and Mandarin (Ottawa) to better understand abiotic stress tolerance. Previous work by our group identified a quantitative trait locus (QTL) on chromosome 5 associated with Fiskeby III iron efficiency, indicating Fiskeby III utilizes iron deficiency stress mechanisms not previously characterized in soybean. We targeted 10 of the potential candidate genes in the Williams 82 genome sequence associated with the QTL using virus-induced gene silencing. Coupling virus-induced gene silencing with RNA-seq, we identified a single high priority candidate gene with a significant impact on iron deficiency response pathways. Characterization of the Fiskeby III responses to iron stress and the genes underlying the chromosome 5 QTL provides novel targets for improved abiotic stress tolerance in soybean.

【 授权许可】

Unknown