Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-Small Cell Lung Cancer

Cancers

Konrad Pawelczyk¹  Agnieszka Gomulkiewicz²  Aleksandra Partynska²  Katarzyna Nowinska²  Karolina Jablonska²  Aleksandra Piotrowska²  Piotr Dziegiel²  Jedrzej Grzegrzolka²  Urszula Ciesielska²  Natalia Glatzel-Plucinska²  Katarzyna Ratajczak-Wielgomas²  Ewa Katnik²  Marzenna Podhorska-Okolow³ 

[1] Department of Thoracic Surgery, Wroclaw Medical University, 53-439 Wroclaw, Poland;Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland;Division of Ultrastructure Research, Wroclaw Medical University, 50-368 Wroclaw, Poland;

关键词: irisin;    ki-67;    ttf-1;    p63;    non-small cell lung cancer;    cancer-associated fibroblasts;    cancer;   

DOI  :  10.3390/cancers11101538

来源: DOAJ

【 摘 要 】

Background: Recent in vitro studies have indicated that irisin inhibits proliferation, migration and epithelial-mesenchymal transition. Irisin expression has not been studied in tumour tissues of non-small cell lung cancer (NSCLC) patients yet. The aim of the study was to determine the irisin expression in NSCLCs in comparison to the clinicopathological factors and expression of TTF-1, p63 and Ki-67. Material and methods: Tissue microarrays with 729 NSCLC and 140 non-malignant lung tissue (NMLT) were used to perform immunohistochemical reactions. Laser Capture Microdissection (LCM) was used to collect cancer and stromal cells from NSCLCs. FNDC5 expression was tested for LCM samples, 75 NSCLCs and 25 NMLTs with the RT-PCR technique. Western-blot, immunofluorescence reaction and RT-PCR assays were performed on lung cancer cell lines. Results: Irisin expression was observed in NSCLC cancer cells and stromal fibroblasts. In cancer cells, irisin expression was decreased in higher grades (G) of malignancy, tumour size (T) and according to lymph node metastasis. In stromal cells, irisin expression was increased in higher G and advanced T. A shorter overall survival was observed in patients with higher irisin expression in NSCLC stromal cells. Conclusions: Irisin expression in stromal fibroblasts may influence cancer cell proliferation and may be a prognostic factor for survival in NSCLC.

【 授权许可】

Unknown