Luteolin, quercetin, genistein and quercetagetin inhibit the effects of lipopolysaccharide obtained from Porphyromonas gingivalis in H9c2 cardiomyoblasts

Cellular & Molecular Biology Letters

Juan Arturo Gómez-Mora¹  Gloria Gutiérrez-Venegas¹  Berenice Fernández-Rojas¹  Alfredo Torras-Ceballos¹ 

[1] Laboratorio de Bioquímica de la División de Estudios de Posgrado de la Facultad de Odontología, Universidad Nacional Autónoma de México Ciudad Universitaria;

关键词: Cardiomyoblasts;    Flavonoids;    Mitogen-activated protein kinase;    Lipopolysaccharide;   

DOI  :  10.1186/s11658-017-0047-z

来源: DOAJ

【 摘 要 】

Abstract Background One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from P. gingivalis promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2). Methods We investigated the effects of luteolin, quercetin, genistein and quercetagetin on cardiomyoblasts treated with LPS alone or in combination with following inhibitors p38 (SB203580), ERK (PD98059), JNK (SP600125) and PKC (Calphostin C) for 1 h. The kinase activation and COX-2 expression levels were determined at the gene and protein levels. Results These flavonoids are considered to inhibit the activation of mitogen-activated protein kinase (MAPK) and the degradation of inhibitor of kappa B-alpha (IκB-α). They also play a role in COX-2 expression. Conclusion We conclude that the tested flavonoids inhibit inflammatory responses induced by LPS in H9c2 cells.

【 授权许可】

Unknown