Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood

Molecular Therapy: Methods & Clinical Development

Patrick J. Hanley¹  Cecilia Barese¹  Hema Dave¹  Min Luo¹  Shabnum Patel¹  Conrad Russell Cruz¹  Catherine M. Bollard¹  J.W. Blaney²  Elizabeth J. Shpall³ 

[1] Center for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USA;Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children’s Hospital, and Houston Methodist Hospital, Houston, TX 77030, USA;Department of Stem Cell Transplantation and Cellular Therapy, MD Anderson Cancer Center, Houston, TX 77030, USA;

关键词: cord blood;    T cells;    adoptive immunotherapy;    cellular therapy;    antiviral T cells;    virus;    cord blood transplantation;   

DOI  :  10.1016/j.omtm.2017.02.001

来源: DOAJ

【 摘 要 】

Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Adoptive transfer of ex vivo-expanded virus-specific T cells from CB is both feasible and safe. However, the manufacturing process of these cells is complicated, lengthy, and labor-intensive. We have now developed a simplified method to manufacture a single culture of polyclonal multivirus-specific cytotoxic T cells in less than 30 days. It eliminates the need for a live virus or transduction with a viral vector, thus making this approach widely available and GMP-applicable to target multiple viruses. The use of overlapping PepMixes as a source of antigen stimulation enable expansion of the repertoire of the T cell product to any virus of interest and make it available as a third party “off the shelf” treatment for viral infections following transplantation.

【 授权许可】

Unknown