Ultra-Performance Liquid Chromatography-Ion Mobility Separation-Quadruple Time-of-Flight MS (UHPLC-IMS-QTOF MS) Metabolomics for Short-Term Biomarker Discovery of Orange Intake: A Randomized, Controlled Crossover Study

Nutrients

Oscar Coltell¹  Carolina Ortega-Azorín²  EvaM. Asensio²  Dolores Corella²  Leticia Lacalle-Bergeron³  JuanVicente Sancho³  FranciscoJ. López³  Tania Portolés³ 

[1] CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain;Department of Preventive Medicine and Public Health, School of Medicine, University of Valencia, 46010 Valencia, Spain;Research Institute for Pesticides and Water (IUPA), Universitat Jaume I, 12071 Castellón, Spain;

关键词: orange intake;    metabolomics;    ion mobility;    biomarkers;   

DOI  :  10.3390/nu12071916

来源: DOAJ

【 摘 要 】

A major problem with dietary assessments is their subjective nature. Untargeted metabolomics and new technologies can shed light on this issue and provide a more complete picture of dietary intake by measuring the profile of metabolites in biological samples. Oranges are one of the most consumed fruits in the world, and therefore one of the most studied for their properties. The aim of this work was the application of untargeted metabolomics approach with the novel combination of ion mobility separation coupled to high resolution mass spectrometry (IMS-HRMS) and study the advantages that this technique can bring to the area of dietary biomarker discovery, with the specific case of biomarkers associated with orange consumption (Citrus reticulata) in plasma samples taken during an acute intervention study (consisting of a randomized, controlled crossover trial in healthy individuals). A total of six markers of acute orange consumption, including betonicines and conjugated flavonoids, were identified with the experimental data and previous literature, demonstrating the advantages of ion mobility in the identification of dietary biomarkers and the benefits that an additional structural descriptor, as the collision cross section value (CCS), can provide in this area.

【 授权许可】

Unknown