Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system

Engineering in Life Sciences

Shogo Miyata¹  Yuuwa Takahashi²  Tetsushi Kiryo² 

[1] Faculty of Science and Technology Keio University Yokohama Kanagawa Japan;Graduate School of Science and Technology Keio University Yokohama Kanagawa Japan;

关键词: cell sorting;    dielectrophoresis;    embryonic stem cell;    pluripotent stem cell;    pluripotency;   

DOI  :  10.1002/elsc.202100113

来源: DOAJ

【 摘 要 】

Abstract Pluripotent stem cells (PSCs) such as embryonic stem cells and induced PSCs can differentiate into all somatic cell types such as cardiomyocytes, nerve cells, and chondrocytes. However, PSCs can easily lose their pluripotency if the culture process is disturbed. Therefore, cell sorting methods for purifying PSCs with pluripotency are important for the establishment and expansion of PSCs. In this study, we focused on dielectrophoresis (DEP) to separate cells without fluorescent dyes or magnetic antibodies. The goal of this study was to establish a cell sorting method for the purification of PSCs based on their pluripotency using DEP and a flow control system. The dielectrophoretic properties of mouse embryonic stem cells (mESCs) with and without pluripotency were evaluated in detail, and mESCs exhibited varying frequency dependencies in the DEP response. Based on the variance in DEP properties, mixed cell suspensions of mESCs can be separated according to their pluripotency with an efficacy of approximately 90%.

【 授权许可】

Unknown