Linearity study on detection and quantification limits for the determination of avermectins using linear regression

Journal of Food and Drug Analysis

Hai Yen Lee¹  Fatimah Abu Bakar¹  Nor Ainy Mahyudin¹  Rafidah Ismail² 

[1] Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia;National Public Health Laboratory, Ministry of Health Malaysia, Lot 1853 Kampung Melayu, 47000 Sg Buloh, Selangor Darul Ehsan, Malaysia;

关键词: Avermectins;    Detection limit;    Dispersive solid-phase extraction;    Linear regression;    Linearity;    Quantification limit;   

DOI  :  10.1016/j.jfda.2014.01.026

来源: DOAJ

【 摘 要 】

In this study, linear relationships between response and concentration were used to estimate the detection limit (DL) and quantification limit (QL) for five avermectins: emamectin, abamectin, doramectin, moxidectin, and ivermectin. Estimation of DL and QL was based on the standard deviation of residual and y-intercept of the regression line at low concentrations of avermectins, using the dispersive solid-phase extraction procedure. Avermectin extracts were analyzed using liquid chromatography tandem mass spectrometry. Based on the regression slope, DL and QL were higher at concentrations of 0.3–0.4 μg/kg and 1 μg/kg, respectively, for all avermectin compounds. Linearity assessment was performed by linear regression, which incorporated a regression model, outlier rejection, and evaluation of the assumption with a significant test. For all avermectins, there is a significant correlation between response and concentration in the range 1–15 μg/kg, and the y-intercept passes through origin (zero).

【 授权许可】

Unknown