Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Medicago Sativa Leaves

Materials

Anna Kiełbasa¹  Katarzyna Rafińska¹  Aneta Krakowska-Sieprawska¹  Bogusław Buszewski¹  Justyna Walczak-Skierska² 

[1] Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St., PL-87100 Torun, Poland;Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wilenska 4 St., PL-87100 Torun, Poland;

关键词: enzymatic hydrolysis;    EA-SFE;    flavonoids;    antioxidant;    HPLC-MS/MS;   

DOI  :  10.3390/ma14112724

来源: DOAJ

【 摘 要 】

To elaborate a complete extraction protocol for the enhanced release of biologically active compounds from plant cells, this study aimed to optimize together the parameters of the supercritical fluid extraction (SFE) process (temperature, pressure, and percentage of cosolvent) and enzymatic treatment of plant material (pH, enzyme concentration, time, and temperature) by response surface methodology (RSM). Medicago sativa L. was selected as a plant material due to its richness in phenolics and flavonoids. HPLC-MS/MS analysis allowed evaluating the content of individual bioactive compounds in obtained extracts. The total content of polyphenolic compounds in the extract obtained after two-step optimization was much higher (546 ± 21 µg/g) than in the extract obtained from non-hydrolyzed material (275 ± 23 µg/g) and in the extract obtained by maceration (162 ± 20 µg/g). Furthermore, it was evidenced that extract with the highest content of polyphenolic compounds can support the cellular antioxidant system both as a free radical scavenger and by stimulating the antioxidant enzyme system.

【 授权许可】

Unknown