The Elongation Factor GmEF4 Is Involved in the Response to Drought and Salt Tolerance in Soybean

International Journal of Molecular Sciences

Jia-Cheng Zheng¹  Jian Ma²  Yong-Bin Zhou³  Jun Chen³  You-Zhi Ma³  Yuan Gao³  Zhao-Shi Xu³  Jin-Dong Fu³  Ming Chen³ 

[1] Anhui Science and Technology University, Fengyang 233100, Anhui, China;College of Agronomy, Jilin Agricultural University, Changchun 130118, China;Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China;

关键词: EF1αs;    genome-wide analysis;    drought tolerance;    salt tolerance;    hairy root assay;    soybean;   

DOI  :  10.3390/ijms20123001

来源: DOAJ

【 摘 要 】

Growing evidence indicates that elongation factor 1α (EF1α) is involved in responses to various abiotic stresses in several plant species. Soybean EF1α proteins include three structural domains: one GTP-binding domain and two oligonucleotide binding domains that are also called as domain 2 and domain 3. In this study, 10 EF1α genes were identified in the soybean genome. We predicted structures of different domains and analyzed gene locations, gene structures, phylogenetic relationships, various cis-elements, and conserved domains of soybean EF1αs. The expression patterns of 10 EF1α genes were analyzed by quantitative real-time PCR (qRT-PCR). Under drought stress, soybean EF1α genes were upregulated in varying degrees. In particular, GmEF4 was upregulated under drought and salt treatments. Compared to the drought- and salt-treated empty vector (EV)-control plants, drought- and salt-treated GmEF4-overexpressing (OE) plants had significantly delayed leaf wilting, longer root, higher biomass, higher proline (Pro) content, and lower H₂O₂, O₂⁻, and malondialdehyde (MDA) contents. Thus, this study provides a foundation for further functional genomics research about this important family under abiotic stress.

【 授权许可】

Unknown