Lactiplantibacillus plantarum dfa1 Outperforms Enterococcus faecium dfa1 on Anti-Obesity in High Fat-Induced Obesity Mice Possibly through the Differences in Gut Dysbiosis Attenuation, despite the Similar Anti-Inflammatory Properties

Nutrients

Suthicha Kanacharoen¹  Natharin Ngamwongsatit²  Asada Leelahavanichkul³  Lampet Wongsaroj⁴  Naraporn Somboonna⁴  Thunnicha Ondee⁵  Kantima Janchot⁵  Krit Pongpirul⁵  Thanapat Lertmongkolaksorn⁶ 

[1] Department of Biology, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD 21218, USA;Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand;Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand;Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;Department of Preventive and Social Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand;Research Management and Development Division, Office of the President, Mahidol University, Nakhon Pathom 73170, Thailand;

关键词: probiotics;    dysbiosis;    obesity;    high fat diet;    Lactiplantibacillus plantarum;    Enterococcus faecium;   

DOI  :  10.3390/nu14010080

来源: DOAJ

【 摘 要 】

Fat reduction and anti-inflammation are commonly claimed properties of probiotics. Lactiplantibacillus plantarum and Enterococcus faecium were tested in high fat-induced obesity mice and in vitro experiments. After 16 weeks of probiotics, L. plantarum dfa1 outperforms E. faecium dfa1 on the anti-obesity property as indicated by body weight, regional fat accumulation, serum cholesterol, inflammatory cytokines (in blood and colon tissue), and gut barrier defect (FITC-dextran assay). With fecal microbiome analysis, L. plantarum dfa1 but not E. faecium dfa1 reduced fecal abundance of pathogenic Proteobacteria without an alteration in total Gram-negative bacteria when compared with non-probiotics obese mice. With palmitic acid induction, the condition media from both probiotics similarly attenuated supernatant IL-8, improved enterocyte integrity and down-regulated cholesterol absorption-associated genes in Caco-2 cell (an enterocyte cell line) and reduced supernatant cytokines (TNF-α and IL-6) with normalization of cell energy status (extracellular flux analysis) in bone-marrow-derived macrophages. Due to the anti-inflammatory effect of the condition media of both probiotics on palmitic acid-activated enterocytes was neutralized by amylase, the active anti-inflammatory molecules might, partly, be exopolysaccharides. As L. plantarum dfa1 out-performed E. faecium dfa1 in anti-obesity property, possibly through the reduced fecal Proteobacteria, with a similar anti-inflammatory exopolysaccharide; L. plantarum is a potentially better option for anti-obesity than E. faecium.

【 授权许可】

Unknown