Screening high‐quality fetal bovine serum for porcine oocyte maturation in vitro

Animal Models and Experimental Medicine

Xueqing Liu¹  Xi Yang¹  Qiling He¹  Xiaoyan You¹  Nan Huang¹  Zijia Liu¹  Puying Xiao¹  Meng Wu¹  Qiaoli Lang¹  Ling Luo¹  Liangpeng Ge¹ 

[1] Chongqing Academy of Animal Sciences Chongqing China;

关键词: fetal bovine serum;    maturation rate;    porcine oocytes;   

DOI  :  10.1002/ame2.12095

来源: DOAJ

【 摘 要 】

Abstract Fetal bovine serum (FBS) is widely used in cell cultures due to its high stability and easy access. It was also used as a substitute for porcine follicular fluid (PFF) in previous studies. However, FBS components are unclear, and the presence of FBS in culture media may introduce a variation from batch to batch. This study aimed to establish an effective method to screen FBS in place of PFF in the culture media for porcine oocytes in vitro. We screened FBS from different sources by using porcine fetal fibroblast cells. The effects of six FBS samples on porcine fetal fibroblast cell growth were tested via frozen cell survival assay, cell clone formation assay, cell growth curve, and cell passage activity assay. The best serum that we called GFBS (heat‐inactivated FBS, cat. no. 10500‐64; Gibco) showed a similar effect on the maturation and development of porcine oocytes to that of PFF and can be used as a good substitute for PFF. These results suggested that the porcine fetal fibroblast cell culture test can be used as a valuable method to screen FBS for porcine oocyte maturation and embryonic development in vitro.

【 授权许可】

Unknown