Candida albicans β-Glucan Differentiates Human Monocytes Into a Specific Subset of Macrophages

Frontiers in Immunology

Sebastian Weis¹  Julia Leonhardt³  Michael Bauer³  Tony Bruns⁴  Sven Stengel⁴  David L. Williams⁵  Fatina Siwczak⁶  Alexander S. Mosig⁶  Michael Kiehntopf⁷  Reinhard Bauer⁸  Silke Große⁸  Regine Heller⁸  Christian Marx⁹  Zhao-Qi Wang⁹ 

[1] Center for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany;Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany;Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital, Jena, Germany;Department of Internal Medicine IV, Jena University Hospital, Jena, Germany;Department of Surgery and Center of Excellence in Inflammation, Infectious Disease and Immunity, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, United States;Institute of Biochemistry II, Jena University Hospital, Jena, Germany;Institute of Clinical Chemistry and Laboratory DiagnosticsJena University Hospital, Jena, Germany;Institute of Molecular Cell Biology, Center for Molecular Biomedicine, Jena University Hospital, Jena, Germany;Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany;

关键词: β-glucan;    Candida albicans;    monocyte survival;    monocyte to macrophage differentiation;    trained immunity;   

DOI  :  10.3389/fimmu.2018.02818

来源: DOAJ

【 摘 要 】

β-Glucan derived from cell walls of Candida albicans is a potent immune modulator. It has been shown to induce trained immunity in monocytes via epigenetic and metabolic reprogramming and to protect from lethal sepsis if applied prior to infection. Since β-glucan-trained monocytes have not been classified within the system of mononuclear phagocytes we analyzed these cells metabolically, phenotypically and functionally with a focus on monocyte-to-macrophage differentiation and compared them with naïve monocytes and other types of monocyte-derived cells such as classically (M1) or alternatively (M2) activated macrophages and monocyte-derived dendritic cells (moDCs). We show that β-glucan inhibits spontaneous apoptosis of monocytes independent from autocrine or paracrine M-CSF release and stimulates monocyte differentiation into macrophages. β-Glucan-differentiated macrophages exhibit increased cell size and granularity and enhanced metabolic activity when compared to naïve monocytes. Although β-glucan-primed cells expressed markers of alternative activation and secreted higher levels of IL-10 after lipopolysaccharide (LPS), their capability to release pro-inflammatory cytokines and to kill bacteria was unaffected. Our data demonstrate that β-glucan priming induces a population of immune competent long-lived monocyte-derived macrophages that may be involved in immunoregulatory processes.

【 授权许可】

Unknown