Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules

Nanomaterials

Balázs Bánhelyi¹  Tibor Novák²  Mária Csete²  Emese Tóth²  Miklós Erdélyi²  Edit Csapó³  Ditta Ungor³  Györgyi Ferenc⁴ 

[1] Department of Computational Optimization, University of Szeged, Árpád Square 2, H-6720 Szeged, Hungary;Department of Optics and Quantum Electronics, University of Szeged, Dóm Square 9, H-6720 Szeged, Hungary;Department of Physical Chemistry and Materials Science, Interdisciplinary Excellence Centre, University of Szeged, Rerrich B. Square 1, H-6720 Szeged, Hungary;Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62, H-6726 Szeged, Hungary;

关键词: gold nanorod;    plasmon resonance;    Cy5 dye molecule;    enhanced fluorescence;    optimization;    DNA;   

DOI  :  10.3390/nano10061048

来源: DOAJ

【 摘 要 】

Plasmonically enhanced fluorescence is a widely studied and applied phenomenon, however, only a comparative theoretical and experimental analysis of coupled fluorophores and plasmonic nanoresonators makes it possible to uncover how this phenomenon can be controlled. A numerical optimization method was applied to design configurations that are capable of resulting in an enhancement of excitation and emission, moreover, of both phenomena simultaneously in coupled Cy5 dye molecule and gold nanorod systems. Parametric sensitivity studies revealed how the fluorescence enhancement depends on the molecule’s location, distance and orientation. Coupled systems designed for simultaneous improvement exhibited the highest (intermediate directional) total fluorescence enhancement, which is accompanied by intermediate sensitivity to the molecule’s parameters, except the location and orientation sensitivity at the excitation wavelength. Gold nanorods with a geometry corresponding to the predicted optimal configurations were synthesized, and DNA strands were used to control the Cy5 dye molecule distance from the nanorod surface via hybridization of the Cy5-labelled oligonucleotide. State-of-the-art dSTORM microscopy was used to accomplish a proof-of-concept experimental demonstration of the theoretically predicted (directional) total fluorescence enhancement. The measured fluorescence enhancement was in good agreement with theoretical predictions, thus providing a complete kit to design and prepare coupled nanosystems exhibiting plasmonically enhanced fluorescence.

【 授权许可】

Unknown