期刊论文详细信息
Frontiers in Bioengineering and Biotechnology
Enzyme-Mediated Lignocellulose Liquefaction Is Highly Substrate-Specific and Influenced by the Substrate Concentration or Rheological Regime
Alexander Sigg1  Timo van der Zwan2  Jinguang Hu2  Jack N. Saddler2  Richard P. Chandra2 
[1] Department of Chemistry, Technical University of Munich, Munich, Germany;Forest Products Biotechnology and Bioenergy Group, Department of Wood Science, Faculty of Forestry, The University of British Columbia, Vancouver, BC, Canada;
关键词: lignocellulose;    enzymatic liquefaction;    high solids;    rheology;    enzymatic hydrolysis;    viscosity;   
DOI  :  10.3389/fbioe.2020.00917
来源: DOAJ
【 摘 要 】

The high viscosities/yield stresses of lignocellulose slurries makes their industrial processing a significant challenge. However, little is known regarding the degree to which liquefaction and its enzymatic requirements are specific to a substrate’s physicochemical and rheological properties. In the work reported here, the substrate- and rheological regime-specificities of liquefaction of various substrates were assessed using real-time in-rheometer viscometry and offline oscillatory rheometry when hydrolyzed by combinations of cellobiohydrolase (Trichoderma reesei Cel7A), endoglucanase (Humicola insolens Cel45A), glycoside hydrolase (GH) family 10 xylanase, and GH family 11 xylanase. In contrast to previous work that has suggested that endoglucanase activity dominates enzymatic liquefaction, all of the enzymes were shown to have at least some liquefaction capacity depending on the substrate and reaction conditions. The contribution of individual enzymes was found to be influenced by the rheological regime; in the concentrated regime, the cellobiohydrolase outperformed the endoglucanase, achieving 2.4-fold higher yield stress reduction over the same timeframe, whereas the endoglucanase performed best in the semi-dilute regime. It was apparent that the significant differences in rheology and liquefaction mechanisms made it difficult to predict the liquefaction capacity of an enzyme or enzyme cocktail at different substrate concentrations.

【 授权许可】

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