Mycobacterium Tuberculosis and Avium Complex In-Vestigation among Malaysian Free-Ranging Wild Boar and Wild Macaques at Wildlife-Livestock-Human In-Terface

Animals

Sabri Jasni¹  Peck Toung Ooi²  Faez Firdaus Abdullah Jesse²  Azlan Che-Amat²  Yusuf Madaki Lekko²  Mazlina Mazlan³  Sharina Omar³  Mohd Firdaus Ariff Abdul-Razak⁴  Siti Zubaidah Ramanoon⁵ 

[1] Department of Paraclinical, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu 16100, Kelantan, Malaysia;Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;Department of Wildlife and National Parks Peninsular Malaysia, Kuala Lumpur 56100, Selangor, Malaysia;Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;

关键词: Mycobacterium tuberculosis complex;    Mycobacterium avium complex;    polymerase chain reaction;    post mortem lesion;    Selangor;    tuberculosis;   

DOI  :  10.3390/ani11113252

来源: DOAJ

【 摘 要 】

Wild animals are considered reservoirs, contributing to the transmission of emerging zoonotic diseases such as tuberculosis (TB). A cross-sectional study was conducted by opportunistic sampling from fresh carcasses of free-ranging wild boar (n = 30), and free-ranging wild macaques (n = 42). Stained smears from these tissues were tested for acid-fast bacilli (AFB) with Ziehl–Neelsen staining. Mycobacterial culture was conducted using Lowenstein–Jensen media and Middlebrook 7H11 agar media. Polymerase chain reaction (PCR) was performed through the detection of the 16S rRNA gene, with multiple sets of primers for the detection of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium avium complex (MAC). In wild boars, 30% (9/30; 95% Confidence Interval: 16.7–47.9%) of examined samples showed gross tuberculosis-like lesions (TBLLs). Multiple nodular lesions that were necrotic/miliary with cavitation were found in the submandibular lymph nodes, tonsils, lungs, kidney and liver, while single nodular lesions were found in the mediastinal lymph nodes, spleen and mesenteric lymph nodes. Conventional PCR on the submandibular lymphoid tissues of wild boar (nine samples with TBLLs and three non-TBLL samples) showed that 75% (9/12) were positive for Mycobacterium bovis (95% CI: 46.8–91.1), and 91% (CI: 64.6–98.5) were positive for Mycobacterium avium. For macaques, 33.3% (10/30) were positive for M. avium (95% CI: 19.2–51.2) but negative for MTBC.

【 授权许可】

Unknown