Serum Levels of Inflammatory Proteins Are Associated With Peripheral Neuropathy in a Cross-Sectional Type-1 Diabetes Cohort

Frontiers in Immunology

Bruce Bode²  Melissa Gardiner³  Shan Bai³  Lynn Kim Hoang Tran³  Wenbo Zhi³  Khaled Bin Satter³  Risa Bernard³  Chandramohan Wakade³  Diane Hopkins³  Jennifer Bryant³  Jin-Xiong She³  Paul Minh Huy Tran³  Mingfang He³  John Morgan⁴  Sharad Purohit⁶  John Chip Reed⁷ 

[1] Diabetes, Atlanta, GA, United States;Atlanta Diabetes Associates, Atlanta, GA, United States;Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA, United States;Department of Neurology, Medical College of Georgia, Augusta University, Augusta, GA, United States;Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta University, Augusta, GA, United States;Department of Undergraduate Health Professionals, College of Allied Health Sciences, Augusta University, Augusta, GA, United States;;Southeastern Endocrine &

关键词: autoimmunity;    chronic inflammation;    type-1 diabetes;    cytokines;    receptors;    peripheral neuropathy;   

DOI  :  10.3389/fimmu.2021.654233

来源: DOAJ

【 摘 要 】

Chronic low-grade inflammation is involved in the pathogenesis of type-1 diabetes (T1D) and its complications. In this cross-section study design, we investigated association between serum levels of soluble cytokine receptors with presence of peripheral neuropathy in 694 type-1 diabetes patients. Sex, age, blood pressure, smoking, alcohol intake, HbA1c and lipid profile, presence of DPN (peripheral and autonomic), retinopathy and nephropathy was obtained from patient’s chart. Measurement of soluble cytokine receptors, markers of systemic and vascular inflammation was done using multiplex immunoassays. Serum levels were elevated in in DPN patients, independent of gender, age and duration of diabetes. Crude odds ratios were significantly associated with presence of DPN for 15/22 proteins. The Odds ratio (OR) remained unchanged for sTNFRI (1.72, p=0.00001), sTNFRII (1.45, p=0.0027), sIL2Rα (1.40, p=0.0023), IGFBP6 (1.51, p=0.0032) and CRP (1.47, p=0.0046) after adjusting for confounding variables, HbA1C, hypertension and dyslipidemia. Further we showed risk of DPN is associated with increase in serum levels of sTNFRI (OR=11.2, p<10), sIL2Rα (8.69, p<10-15), sNTFRII (4.8, p<10-8) and MMP2 (4.5, p<10-5). We combined the serum concentration using ridge regression, into a composite score, which can stratify the DPN patients into low, medium and high-risk groups. Our results here show activation of inflammatory pathway in DPN patients, and could be a potential clinical tool to identify T1D patients for therapeutic intervention of anti-inflammatory therapies.

【 授权许可】

Unknown