Modular Breath Analyzer (MBA): Introduction of a Breath Analyzer Platform Based on an Innovative and Unique, Modular eNose Concept for Breath Diagnostics and Utilization of Calibration Transfer Methods in Breath Analysis Studies

Molecules

Carsten Jaeschke¹  Johannes Glöckler¹  Boris Mizaikoff¹  Inese Polaka²  Viktors Veliks²  Martins Leja²  Marcis Leja²  Marta Padilla³  Jan Mitrovics³ 

[1] Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany;Institute of Clinical and Preventive Medicine, University of Latvia, LV-1079 Riga, Latvia;JLM Innovation GmbH, Vor dem Kreuzberg 17, 72070 Tuebingen, Germany;

关键词: breath analysis;    MOX sensors;    low sensing chamber volume;    calibration transfer;    standard samples;    piecewise direct standardization;   

DOI  :  10.3390/molecules26123776

来源: DOAJ

【 摘 要 】

Exhaled breath analysis for early disease detection may provide a convenient method for painless and non-invasive diagnosis. In this work, a novel, compact and easy-to-use breath analyzer platform with a modular sensing chamber and direct breath sampling unit is presented. The developed analyzer system comprises a compact, low volume, temperature-controlled sensing chamber in three modules that can host any type of resistive gas sensor arrays. Furthermore, in this study three modular breath analyzers are explicitly tested for reproducibility in a real-life breath analysis experiment with several calibration transfer (CT) techniques using transfer samples from the experiment. The experiment consists of classifying breath samples from 15 subjects before and after eating a specific meal using three instruments. We investigate the possibility to transfer calibration models across instruments using transfer samples from the experiment under study, since representative samples of human breath at some conditions are difficult to simulate in a laboratory. For example, exhaled breath from subjects suffering from a disease for which the biomarkers are mostly unknown. Results show that many transfer samples of all the classes under study (in our case meal/no meal) are needed, although some CT methods present reasonably good results with only one class.

【 授权许可】

Unknown