Nanoscale organization of rotavirus replication machineries

eLife

Haydee Olinca Hernández¹  Susana López¹  Mayra Méndez¹  Adán Guerrero²  Juan Manuel Rendon-Mancha²  Jose L Martínez³  Christopher D Wood³  Daniela Silva-Ayala³  David Torres Hernández³  Arianna Pérez-Delgado³  Carlos F Arias³  Yasel Garcés Suárez³ 

[1] Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico;Centro de Investigación en Ciencias, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico;Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico;

关键词: super resolution microscopy;    rotavirus;    viroplasms;   

DOI  :  10.7554/eLife.42906

来源: DOAJ

【 摘 要 】

Rotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins and their organization in VPs have lacked molecular-scale spatial resolution, due to inherent spatial resolution constraints. In this work we employed super-resolution microscopy to reveal the nanometric-scale organization of VPs formed during rotavirus infection, and quantitatively describe the structural organization of seven viral proteins within and around the VPs. The observed viral components are spatially organized as five concentric layers, in which NSP5 localizes at the center of the VPs, surrounded by a layer of NSP2 and NSP4 proteins, followed by an intermediate zone comprised of the VP1, VP2, VP6. In the outermost zone, we observed a ring of VP4 and finally a layer of VP7. These findings show that rotavirus VPs are highly organized organelles.

【 授权许可】

Unknown