Vitamin C and E Treatment Blunts Sprint Interval Training–Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans

Antioxidants

BrigitteA. Graf¹  Stefano Gastaldello²  VictoriaL. Wyckelsma²  Sigitas Kamandulis²  Reedik Pääsuke³  Bengt Kayser⁴  Albertas Skurvydas⁵  Håkan Westerblad⁵  Audrius Snieckus⁵  Neringa Baranauskiene⁵  Marius Brazaitis⁵  Tomas Venckunas⁵  Andrejus Subocius⁵  Nerijus Eimantas⁵  Helena Gapeyeva⁶  Mati Pääsuke⁶  DanielC. Andersson⁷  Nicolas Place⁷  Priit Kaasik⁸  Jaak Jürimäe⁹ 

[1] University of Tartu, 50090 Tartu, Estonia;Department of Physiology and Pharmacology, Karolinska Institutet, 171 77 Stockholm, Sweden;Department of Traumatology and Orthopaedics, Tartu University Hospital, 50090 Tartu, Estonia;Food and Nutrition Department of Health Professions, Faculty of Health, Manchester Metropolitan University, Manchester M1 5GF, UK;Institute of Sport Science and Innovations, Lithuanian Sports University, 44221 Kaunas, Lithuania;Institute of Sport Sciences and Physiotherapy, University of Tartu, 50090 Tartu, Estonia;Institute of Sports Sciences, University of Lausanne, 1015 Lausanne, Switzerland;Laboratory of Functional Morphology, University of Tartu, 50090 Tartu, Estonia;Laboratory of Kinanthropometry;

关键词: sprint interval training;    high-intensity interval training;    aging;    endurance exercise;    skeletal muscle;    antioxidant treatment;   

DOI  :  10.3390/antiox9090879

来源: DOAJ

【 摘 要 】

Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4-6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)- and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca²⁺ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.

【 授权许可】

Unknown