The Intraocular Pressure-Lowering Effect of Persimmon leaves (Diospyros kaki) in a Mouse Model of Glaucoma

International Journal of Molecular Sciences

Tae-Jin Kim¹  ChangYong Lee²  HongRyul Ahn³  SangHoon Jung³  JeeYoung Kim⁴  JaeWook Yang⁴ 

[1] Department of Biological Sciences, Pusan National University, Busan 46241, Korea;Department of Food Science, Cornell University, Ithaca, NY 14850, USA;Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Korea;T2B Infrastructure Center for Ocular disease, Department of Ophthalmology, Inje University Busan Paik Hospital, Busan 47392, Korea;

关键词: persimmon leaves;    ocular hypertension;    intraocular pressure;    soluble guanylate cyclase;    glaucoma;   

DOI  :  10.3390/ijms20215268

来源: DOAJ

【 摘 要 】

The aim of this study was to evaluate the pharmacological efficacy of persimmon leaves in two glaucoma models, microbeads-induced ocular hypertension (OHT) and DBA/2 mouse. Thus, we demonstrated that Ethanol Extract of Diospyros kaki (EEDK) reduced elevated intraocular pressure (IOP) in both mouse models of glaucoma by measurements with a tonometer. In particular, we revealed that retinal ganglion cell loss and optic nerve damage caused by IOP elevation were markedly diminished as assessed by TUNEL assay, H&E staining, and fluorescent staining, while the expression of soluble guanylate cyclase (sGCα-1) increased, when EEDK was administered, as revealed by western blot. Moreover, the b-wave magnitude indicating functional scotopic vision was significantly improved in EEDK-administered DBA/2 mice during the 10-week follow-up study, as observed with electroretinography. Collectively, our results suggested that EEDK could be an effective therapeutic and IOP-lowering agent for preventing and treating retinal degenerative diseases such as glaucoma.

【 授权许可】

Unknown