Stable in Biocompatible Buffers Silver Nanoisland Films for SERS

Biosensors

Alexey Skvortsov¹  Ekaterina Babich²  Andrey Lipovskii²  Valentina Zhurikhina²  Alexey Redkov³ 

[1] Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, Russia;Institute of Physics and Mechanics, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, Russia;Institute of Problems of Mechanical Engineering, Bolshoy pr. V. O. 61, 199178 St. Petersburg, Russia;

关键词: ion-exchange;    silver nanoparticles;    stability;    luminescence;    Raman;    SERS;   

DOI  :  10.3390/bios11110448

来源: DOAJ

【 摘 要 】

We investigated the stability of silver nanoisland films, which were formed on glass surface by the method of out-diffusion, in biocompatible buffers and the applicability of the films in surface enhanced Raman scattering (SERS). We have shown that silver nanoisland films are stable in one of the most widespread in biological studies buffer—phosphate buffer saline (PBS), and in 1:100 water-diluted PBS, in the PBS-based buffer, in which NaCl is replaced by the same amount of NaClO₄, and in acidic phosphate buffer. At the same time, the replacement of NaCl in PBS by N(CH₃)₄Cl leads to the degradation of the nanoislands. It was shown that after exposure to PBS the nanoisland films provided a good SERS signal from a monolayer of 1,2-di(4-pyridyl)ethylene (BPE), which makes silver nanoisland films promising for biosensor applications. Additionally, in our experiments, we registered for the first time that silver nanoparticles formed in the bulk of the samples dissolved after exposing to PBS, while nanoislands on the glass surface stayed unchanged. We associate this phenomenon with the interaction of ions contained in PBS solution with silver, which results in the shift of corresponding chemical equilibrium.

【 授权许可】

Unknown