Microstructural, Electrochemical and In Vitro Analysis of Mg-0.5Ca-xGd Biodegradable Alloys

Applied Sciences

Ramona Cimpoesu¹  Nicanor Cimpoesu¹  MariaDaniela Vlad²  OanaDiana Popescu³  Corneliu Munteanu³  Bogdan Istrate³ 

[1] Faculty of Material Science and Engineering Department, Gheorghe Asachi University of Iasi, 41 Dimitrie Mangeron Str., 700050 Iasi, Romania;Faculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy from Iasi, 9-13 Kogălniceanu Str., 700454 Iasi, Romania;Mechanical Engineering Department, Gheorghe Asachi University of Iasi, 6 D. Mangeron Blvd, 700050 Iasi, Romania;

关键词: Mg–Ca–Gd alloys;    SEM;    EDS;    microstructure;    electrochemical evaluation;    in vitro test;   

DOI  :  10.3390/app11030981

来源: DOAJ

【 摘 要 】

The subject of Mg-based biodegradable materials, used for medical applications, has been extensively studied throughout the years. It is a known fact that alloying Mg with biocompatible and non-toxic elements improves the biodegradability of the alloys that are being used in the field of surgical applications. The aim of this research is to investigate the aspects concerning the microstructure, electrochemical response (corrosion resistance) and in vitro cytocompatibility of a new experimental Mg-based biodegradable alloy—Mg–0.5%Ca with controlled addition of Gd as follows: 0.5, 1.0, 1.5, 2.0 and 3.0 wt.%—in order to establish improved biocompatibility with the human hard and soft tissues at a stable biodegradable rate. For this purpose, scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), light microscopy (LM) and X-ray diffraction (XRD) were used for determining the microstructure and chemical composition of the studied alloy and the linear polarization resistance (LPR) method was used to calculate the corrosion rate for the biodegradability rate assessment. The cellular response was evaluated using the 3-(4,5-dimethyltiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test followed by fluorescence microscopy observation. The research led to the discovery of a dendritic α-Mg solid solution, as well as a lamellar Mg₂Ca and a Mg₅Gd intermetallic compound. The in vivo tests revealed 73–80% viability of the cells registered at 3 days and between 77 and 100% for 5 days, a fact that leads us to believe that the experimental studied alloys do not have a cytotoxic character and are suitable for medical applications.

【 授权许可】

Unknown