Recent Advances in Additive Manufacturing and 3D Bioprinting for Organs-On-A-Chip and Microphysiological Systems

Frontiers in Bioengineering and Biotechnology

Eva I. Reihs¹  Reinhard Windhager²  Sarah Spitz⁴  Peter Ertl⁴  Sebastian R. A. Kratz⁴  Mario Rothbauer⁶  Stefan Toegel⁶  Christoph Eilenberger⁷  Barbara E. M. Bachmann⁸ 

[1] Austrian Cluster for Tissue Regeneration, Vienna, Austria;Division of Orthopedics, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Vienna, Austria;Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria;Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria;Karl Chiari Lab for Orthopaedic Biology, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Vienna, Austria;Ludwig Boltzmann Institute for Arthritis and Rehabilitation, Vienna, Austria;Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Centre, Vienna, Austria;Ludwig Boltzmann Research Group Senescence and Healing of Wounds, Vienna, Austria;

关键词: bioprinting application;    organs- and tissues-on-a-chip;    Microphysiological systems;    STL;    2PP;    droplets;   

DOI  :  10.3389/fbioe.2022.837087

来源: DOAJ

【 摘 要 】

The re-creation of physiological cellular microenvironments that truly resemble complex in vivo architectures is the key aspect in the development of advanced in vitro organotypic tissue constructs. Among others, organ-on-a-chip technology has been increasingly used in recent years to create improved models for organs and tissues in human health and disease, because of its ability to provide spatio-temporal control over soluble cues, biophysical signals and biomechanical forces necessary to maintain proper organotypic functions. While media supply and waste removal are controlled by microfluidic channel by a network the formation of tissue-like architectures in designated micro-structured hydrogel compartments is commonly achieved by cellular self-assembly and intrinsic biological reorganization mechanisms. The recent combination of organ-on-a-chip technology with three-dimensional (3D) bioprinting and additive manufacturing techniques allows for an unprecedented control over tissue structures with the ability to also generate anisotropic constructs as often seen in in vivo tissue architectures. This review highlights progress made in bioprinting applications for organ-on-a-chip technology, and discusses synergies and limitations between organ-on-a-chip technology and 3D bioprinting in the creation of next generation biomimetic in vitro tissue models.

【 授权许可】

Unknown