Antimicrobial activity of endophytic fungi from coffee plants

Bioscience Journal

Sara Maria Chalfoun de Souza¹  Natálie Martins Alves²  Patrícia Gomes Cardoso²  Mônica Cristina Pereira Monteiro²  Olinto Liparini Pereira³  Marisa Vieira de Queiroz⁴  Danilo Batista Pinho⁵ 

[1] Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG;Universidade Federal de Lavras - UFLA;Universidade Federal de Viçosa -UFV;Universidade Federal de Viçosa- UFV;Universidade de Brasília ;

关键词: aspergillus ochraceus;    fusarium verticillioides;    inhibition;    muscodor spp.;    volatiles compounds.;   

DOI  :  10.14393/BJ-v33n2-34494

来源: DOAJ

【 摘 要 】

Endophytic fungi are a promising source for discovery of compounds with biotechnological potential. The aim of this study was to select and identify endophytic fungi from Coffea arabica that produce volatile organic compounds (VOCs), evaluate the effect of the VOCs produced by endophytic fungi on the growth of Rhizoctonia solani, Fusarium oxysporum, Phoma sp., Botrytis cinerea, Fusarium solani, Fusarium verticillioides, Cercospora coffeicola and Pestalotia longisetula, and select endophytic fungi with potential for biological control of Aspergillus ochraceus inoculated in coffee beans and F. verticillioides inoculated in corn seeds. An isolate of Muscodor albus was used as selection tool for VOC producing fungi. Among the 400 endophytic fungi isolates, 11 were able to grow in the presence of VOCs produced by M. albus. These fungi were identified as Muscodor spp. (9) and Simplicillium sp. according to searches in UNITE database using DNA sequences of internal transcribed spacer (ITS). The VOC's produced by endophytic fungi inhibited the growth the phytopathogenic fungi with different efficacies, compared to the control. The VOCs produced by Muscodor coffeanum (COAD 1842) showed fungicidal effect against A. ochraceus on coffee beans. Six endophytic fungi completely inhibited growth of F. verticillioides inoculated in corn seeds. This study demonstrates that the volatile-compound producing endophytic fungi, isolated from Coffea arabica, are promising sources of bioactive compounds.

【 授权许可】

Unknown